基于高通量测序技术对沙湖水体细菌多样性的分析

Bacterial diversity analysis in Shahu Lake based-on high-throughput sequencing

1. College of Biological Science & Engineering, Beifang Univesity of Nationality, Yinchuan 750021；2. Inner Mongolia Academy of Environmental Sciences, Hohhot 010010； 3. College of Environment ＆ Resources, Inner Mongolia University， Hohhot 010021, China

Online:2017-12-18 Published:2017-12-18

Abstract

Abstract: In order to investigate the structure and diversity of microbial community in Shahu Lake, the total DNA of bacteria from water samples was extracted by E.Z.N.A. Water DNA extraction kit, then the V4-V5 region of 16S rRNA gene was sequenced by MiSeq technology. Mothur software and R language were used to analyze the microbial community structure and diversity. A total of 427975 optimized sequences were obtained, and 20 Phylum, 50 Class, 95 Order, 161 Family, and 276 Genus were predicted at the 97% similarity level. The OTUs for A, B, C and D were 497, 496, 487 and 511, respectively. Proteobacteria, Cyanobacteria, Actinobacteria and Bacteroidetes are dominant groups. The microbial community structure in Shahu Lake is complex, but the composition and diversity of microbial community structure are not significant at different sites.

Key words: microbial communities, Shahu Lake, water, MiSeq sequencing

ZHANG Yan-ling, LI Jing-yu, LIU Jian-li, LIU Ya-qin,ZHANG Yi-fei, ZHAO Ji, DENG Wei. Bacterial diversity analysis in Shahu Lake based-on high-throughput sequencing[J]. .

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Bacterial diversity analysis in Shahu Lake based-on high-throughput sequencing

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