Abstract: Diethylaminoethyl(DEAE)-Agarose was studied for preparing monolayers based on the Langmuir-Blodgett(LB) film technology. A high collapse pressure and a standard film forming process were found in the surface pressure (π)-area (A) isotherm of DEAE-Agarose. The effect of DEAE-Agarose concentration on the film formation showed that 0.5 mg/mL was the best. The surface potential increased with rise of the ligand density. DEAE-Agarose LB films were characterized with Brewster angle microscopy (BAM) and atomic force microscope (AFM) after being transferred onto silicon surface. The morphology of the film was pretty compact, well structured, and relatively smooth. The thickness was about 7.9 nm and the roughness was about 2 nm. This result indicated that the DEAE-Agarose LB film was successfully prepared and could be an novel material for the fabrication of LB films.

Key words: DEAE-Agarose, Langmuir-Blodgett film, atomic force microscope, Brewster angle microscopy, surface potential