Abstract: The mechanism of nanosecond pulse dielectric barrier discharge plasma (NS-DBD) promoting the growth ofGanoderma lucidumand the biosynthesis of ganoderic acids from the perspective of epigenetics was explored. Through high-performance liquid chromatography detection, it was found that NS-DBD can promote the biosynthesis of ganoderic acids. Through biochemical indicators detection, it was found that NS-DBD induced the accumulation of intracellular reactive oxygen species inG. lucidum, leading to an increase in the activity of antioxidant enzymes such as total superoxide dismutase, catalase, and glutathione reductase. Simultaneously, there was an increase in the expression of genes related to the biosynthesis of ganoderic acids. Through immunofluorescence and chromatin immunoprecipitation sequencing methods, it was confirmed that there were significant changes in the H3K4me2 modification levels of geneshmgr, pmvk, mvd, sqs, andlsin the ganoderic acids synthesis pathway after NS-DBD treatment. The H3K4me2 modifications of geneshmgr, pmvk, mvd, andlswere mainly distributed in the exon regions of the genes, while the H3K4me2 modifications of genesqswere distributed in the upstream 2 kb and exon regions. Therefore, this study suggested that NS-DBD regulates the H3K4me2 modification levels of some key genes in the ganoderic acids synthesis pathway by stimulating ROS accumulation, thereby regulating the growth ofG. lucidumand the synthesis of ganoderic acids.

Key words: low-temperature plasma, dielectric barrier discharge (DBD);Ganoderma lucidum, ganoderic acids, stimulation effect, H3K4me2