Abstract: High efficiency phosphor-solublePseudomonassp. 1616X1 was used as the object and Ca3(PO4)2was used as the phosphorus source to explore the mechanism ofPseudomonasin dissolving insoluble inorganic phosphorus. The kinds and content of organic acids in the process of solubilizing phosphorus were determined by using high performance liquid chromatography (HPLC), the complete genome ofPseudomonassp. 1616X1 was sequenced by using the Illumina HiSeq X Ten sequencing platform, gene prediction, functional annotation, and analysis of phosphorus-solubilizing related genes were completed after sequence assembly. The results showed thatPseudomonassp. 1616X1 produced gluconic acid, tartaric acid, citric acid and other organic acids in the process of phosphorus dissolution, among which gluconic acid was the main one. The content of gluconic acid reached 17582.06 mg/L when the maximum amount of phosphorus was dissolved. The complete genome analysis revealed that the gene length ofPseudomonassp. 1616X1 was 5957 620 bp with 61.55% overall GC content, including 5535 coding sequences. Based on comparison and annotation by database of COG, GO and KEGG, the strain contained pyrroloquinoline quinone biosynthesis gene cluster (pqq), phosphate transport system synthesis genes (pstandpit) and phosphorus starvation response regulation genes (phoB,phoRandphoH). And it contained glucose dehydrogenase gene (gdh), pyrroloquinoline quinone-dependent glucose dehydrogenase gene (gcd), citrate synthase gene (gltA) and tartrate dehydrogenase gene (ttuC) and other organic acid synthesis genes. The findings of this study provided a foundation for understanding the mechanism of phosphate solubilization ofPseudomonassp. 1616X1 and future genetic modification research.

Key words: Pseudomonas, soil phosphorus solution, organic acids, genome analysis, phosphate solubilizing genes