Abstract: The study determined the effect of charcoal medicine extract (liquid dressing 1 and liquid dressing 2) on the changes of type I and III collagen content in the healing process of skin wounds in mice. A model of full-thickness wound in mice was established. Liquid dressing 1 and liquid dressing 2 were applied daily on the back of mice. After 3, 6, 9, 13, 17 and 21 days of administration, the content of collagen I and III in wound tissues of mice was determined by HPLC-MS. Results showed that liquid dressing 1 and liquid dressing 2 can obviously improve the wound healing rate and accelerate the scab shedding. Compared with the control group, liquid dressing 1 and liquid dressing 2 could obviously promote the synthesis of total collagen at 9 d and 13 d(P<0.01). The content of type I and type III collagen in liquid dressing 1 and liquid dressing 2 were greater than that of control group at 3 d and 9 d (P< 0.05). The ratio of collagen I/III in liquid dressing 1 and liquid dressing 2 were greater than that of control group at 17 d and 21 d (P<0.01). This study provided a theoretical basis for the treatment of wound healing with charcoal medicine extract.

Key words: charcoal medicine extract, wound healing, type I collagen, type III collagen, fibroblast