Abstract: Type I and III collagen are the major components of porcine small intestine submucosal extracellular matrix (SIS). This study intends to establish a collagen type identification and quantitative detection method based on liquid chromatography-mass spectrometry. Firstly, the ion trap mass spectrometry (LCQ) was used to detect the digestion of the collagen standard and SIS products that had been marketing to identify the type of collagen and its marker peptides. The marker peptide of type I collagen was GETGPAGPAGPVGPVGAR, and type III was GPPGAVGPSGPR. The triple quadrupole mass spectrometry (TSQ) was used to detect the marker peptides, and then the contents of type I and III collagen in SIS were calculated by the linear relationship between the concentration and the peak area. The result showed that the contents of I and III in VIDASISTM and Biodesign were close to that of the total collagen content. the contents of I and III collagen in porcine Achilles tendon and dermis were detected by HPLC-MS, and the results accorded with the distribution of type I and III collagen in tissues.

Key words: porcine small intestinal submucosa, acellular matrix, type Ⅰ collagen, type III collagen, HPLC-MS