生物学杂志

Journal of Biology ›› 2023, Vol. 40 ›› Issue (1): 34-.doi: 10.3969/j.issn.2095-1736.2023.01.034

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Genome sequencing and analysis of a highly efficient  oil-degrading bacterium Falsochrobactrum TDYN1

CHEN Guanyi1,2, CHEN Hongyun1, LI Gaoyuan1, HU Mengjie3, LI Xinhao1，ZHONG Lei1   

  1. 1. School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China;
    2. School of Mechanical Engineering, Tianjin University of Commerce, Tianjin 300134, China;
    3. School of Biotechnology and Food Science, Tianjin University, Tianjin 300134, China
  • Online:2023-02-18 Published:2023-02-21

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Abstract: Falsochrobactrum TDYN1 was isolated from the Dagang oilfield in Tianjin. Based on lllumina Hiseq2000, the most widely used second-generation sequencing platform, TDYN1 was de novo sequenced. The basic function of GO, KEGG, COG, NR and Swiss-Prot databases was annotated. The results indicated that the size of the TDYN1 genome was 3076677 bp with 23 Scaffolds, and an average of GC content at 55.96%, and 3465 genes were successfully predicted, which 1689, 1946, 2998, 2513, 2841 and 3197 genes could be matched with annotation information in the GO, KEGG, COG, Swiss-Prot and NR databases respectively. At the same time, it was predicted that TDYN1 had pathways related to petroleum hydrocarbon metabolism, and the ability to degrade dioxins, polycyclic aromatic hydrocarbons and so on. Genome sequencing and analysis of TDYN1 laid an important basis for further research on the mechanism and application of petroleum hydrocarbon degradation of TDYN1, and provided theoretical data for the production of functional bacteria.

Key words: petroleum degrading bacterium, Falsochrobactrum TDYN1, genome sequencing, gene function annotation

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