Abstract: To analyse the effect of dodecyl hydroxypropyl sulfobetaine（DSB）on bacterial metabolism，clearance efficiency and formation of polysaccharide intercellular adhesion ofStaphylococcusepidermidisbiofilm and to provide reliable theory and practical evidence for DSB to prevent biofilm contamination ofS.epidermidis，vancomycin was used as positive control，XTT reduction was used to evaluate the clearance and metabolism of DSB onS.epidermidisbiofilm，Congo red medium and laser scanning confocal microscope（LSCM）were used to observe the effect of DSB on PIA and biofilm，respectively. The results showed that 1 024，512，256 and 128 mg/L of DBS had significantly effect on metabolism of ATCC 35984，and 64，32，16 and 8 mg/L of DBS had significantly effect on clearance in the 6，12and24 hours post treatment（P<0.001）. However，16 and 8 mg/L of DSB had no significant effect on PIA formation. These results indicated that DSB significantly affects bacterial metabolism in biofilms ofS.epidermidisand in scavenging formed biofilm.

Key words: dodecyl hydroxypropyl sulfobetaine, Staphylococcusepidermidis, biofilm, metabolism, polysaccharide intercellular adhesion（PIA）, clearance