Abstract: The VP3 gene was inserted into the eukaryotic expression vector pEGPF-N2, and further transfected the recombinant plasmid into MA104 cells to observe whether the expressed VP3 has an effect on rotavirus replication. The expression of recombinant protein was detected by immunofluorescence and Western Blot. The expression of VP3 was the strongest at 48 h post transfection in MA104 cells. And the replication of the virus was detected by qRT-PCR and immunofluorescence. The results showed that the recombinant protein VP3 could promote viral replication, which provided an experimental basis for further exploration of the function of VP3 and its role in rotavirus replication.

Key words: rotavirus, eukaryotic expression vector, VP3, virus replication, antiviral response