生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (2): 51-.doi: 10.3969/j.issn.2095-1736.2021.02.051

• 研究报告 • 上一篇    下一篇

水稻草铵膦耐性突变体的筛选及其耐性机制

  

  1. 1.中国科学院合肥物质科学研究院技术生物与农业工程研究所，合肥230031； 2.中国科学技术大学，合肥230026； 3.安徽农业大学农学院，合肥230036
  • 出版日期:2021-04-18 发布日期:2021-04-25
  • 通讯作者: 吴跃进，研究员，博士生导师，主要从事物理与生物技术交叉研究，Email:yjwu@ipp.ac.cn;刘斌美，副研究员，硕士生导师，主要从事水稻诱变及分子育种研究，Email:liubm@ipp.ac.cn
  • 作者简介:任艳，硕士研究生，研究方向为水稻突变体的筛选及机制研究
  • 基金资助:
    安徽省科技重大专项（18030701205）；国家自然科学基金（31701330）；中国科学院科技服务网络计划（ST计划，KFJ-STS-ZDTP-054）

Screening of rice（Oryza sativa L.）glufosinate⁃tolerant mutants and preliminary study on its mechanism

  1. 1. Institute of Technical Biology and Agriculture Engineering，Hefei Institutes of Physical Science，Chinese Academy of Sciences，Hefei 230031，China； 2. University of Science and Technology of China，Hefei 230026，China； 3. College of Agronomy，Anhui Agricultural University，Hefei 230036，China
  • Online:2021-04-18 Published:2021-04-25

PDF (PC)

103

摘要： 以水稻品种“金粳818”为诱变材料，进行水稻草铵膦耐性突变体筛选和耐性生理指标测定。从重离子辐照后代中获得5个草铵膦抗性的材料，进一步的草铵膦涂抹研究结果表明：处理96 h后草铵膦耐性突变体水稻叶片仍保持绿色，对照组叶片完全枯黄萎蔫，叶绿素含量相对值显著高于对照组。突变系水稻鲜重、干重和株高比对照组最高分别增加111.46%、53.40%和48.00%。突变系水稻叶片氨含量均低于0.8 mg/g，而对照组氨含量高达1.3 mg/g。草铵膦耐性突变体与对照组的叶片谷氨酰胺合成酶活性均下降，但两者差异不显著，而突变系水稻的草铵膦耐性机制是否是非靶标抗性还需进一步验证。筛选出的草铵膦耐性突变体水稻具有一定的草铵膦耐性，在草铵膦耐性分子机制研究或育种方面具有良好前景。

关键词: 水稻, 草铵膦, 叶绿素, 谷氨酰胺合成酶, 咪唑乙烟酸

Abstract: One rice variety，“Jingeng818”，was applied as material to screen glufosinate⁃tolerant（GT）rice mutants using heavy ion irradiation technique. Some physiological indicators of glufosinate tolerance were determined on the selected mutants and control rice groups after glufosinate spraying. The results showed that the leaf color of GT mutants maintained green with a significant higher chlorophyll content，while the leaves in control group were withered with lower chlorophyll content. After glufosinate spraying，the fresh biomass weight，dry weight and plant height of the GT rice mutants increased by 111.46%，53.40% and 48.00%，respectively，compared with those of the control group. The leaf ammonia content displayed the value lower than 0.8 mg/g in GT rice mutants，but the value was up to 1.3 mg/g in control. The glutamine synthase activities decreased both in the mutant rice and the control group，and there was no significant difference between the two treatments. Whether the glufosinate-tolerance mechanism of mutant rice is non-target resistance needs further verification.In conclusion，the screened GT rice mutants demonstrate a certain tolerance to glufosinate，and present huge potential in revealing molecular mechanism of glufosinate tolerance and variety breeding.

Key words: rice, glufosinate, chlorophyll, glutamine synthase, imazamox

中图分类号: