生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (3): 63-.doi: 10.3969/j.issn.2095-1736.2025.03.063

• 研究报告 • 上一篇    下一篇

Somatostatin 4基因在斑马鱼发育过程中的定位及其功能

高　静1，2， 袁辉明1，2， 刘　璐1，2， 聂　婷1，2， 谭炜波1，2， 陈　洁1，2   

  1. 1. 上海海洋大学 国家海洋生物科学国际联合研究中心， 上海 201306;
    2. 上海海洋大学 水产种质资源发掘与利用教育部重点实验室， 上海 201306
  • 出版日期:2025-06-18 发布日期:2025-06-16
  • 通讯作者: 陈洁，博士，助理研究员，研究方向为鱼类内分泌，E-mail:chenjie@shou.edu.cn
  • 作者简介:高静，硕士，研究方向为鱼类内分泌，E-mail:freya0512@163.com；袁辉明，硕士，研究方向为鱼类内分泌，E-mail:yhm66z@163.com；高静和袁辉明为共同第一作者
  • 基金资助:
    上海海洋大学水产一流学科支持项目(A1-3201-19-3020)

Localization and function of somatostatin 4 in the development of zebrafish

GAO Jing1,2, YUAN Huiming1,2, LIU Lu1,2, NIE Ting1,2, TAN Weibo1,2, CHEN Jie1,2   

  1. 1. International Research Center for Marine Biosciences, Shanghai Ocean University, Shanghai 201306, China;
    2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education,
    Shangahi Ocean Univeristy, Shanghai 201306, China
  • Online:2025-06-18 Published:2025-06-16

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摘要： 为了研究生长抑素基因（somatostatin 4， sst4）在斑马鱼（Danio rerio）发育过程中的表达分布与主要功能，通过原位杂交技术确定斑马鱼胚胎受精后24~96 h胰腺中sst4基因的特异表达。葡萄糖处理实验显示，在高葡萄糖质量浓度下5日龄斑马鱼中sst4基因表达下调，暗示其在胰腺发育分化和血糖调控中的重要作用。然后，分别对产生胰高血糖素的α细胞和产生胰岛素的β细胞进行可视化计数后进行基因型鉴定，结果显示，sst4基因敲除导致β细胞明显增多，而α细胞并未受到影响。120日龄成鱼血糖检测结果显示，sst4突变体血糖水平降低，与此同时，葡萄糖代谢相关基因insa和insb表达下调，gcgb表达量上调，g6pca.1表达量下降。结果表明，sst4基因定位于胰腺，对胰腺β细胞的增殖具有抑制作用，并且可能通过作用于β细胞参与调控血糖稳态。

关键词: 生长抑素, 胰腺, β细胞增殖, 血糖, 斑马鱼

Abstract: In order to investigate the distribution and function ofsomatostatin 4 (sst4) gene during zebrafish (Danio rerio) development,sst4gene specifically expressed in the pancreas of zebrafish was identified during 24－96 hpf byin situhybridization (ISH). By espousing the 5 dpf zebrafish larva with different mass concentration of glucose, qPCR analysis showed thatsst4expression levels were significantly reduced with the increase of the exposed glucose concentration. These results suggested an important role ofsst4gene on pancreatic development and differentiation and glucose regulation. Further, by counted the number of α cells producing glucagon and β cells producing insulin, laser confocal scanning results showed that in thesst4mutant, β cell number was significantly increased compared to that in the wildtype fish, while α cell number was no difference between the two genotypes. Blood glucose measurement in adults (120 dpf) showed thatsst4mutants were with lower glucose levels, compared to wildtype. And glucose metabolism related genes,insa,insbandg6pca.1showed a significant decrease, whilegcgbgene showed an increase in thesst4mutant. These results indicated that thesst4gene may involve into blood glucose homeostasis though its function as an inhibitor of β cell proliferation.

Key words: somatostatin 4, pancreas, β cell proliferation, blood glucose, zebrafish

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