生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 38-.doi: 10.3969/j.issn.2095-1736.2023.02.038

• 研究报告 • 上一篇    下一篇

内蒙古岱海中细菌多样性分析

姜　凯1,2, 曹春玲3   

  1. 1. 内蒙古师范大学 生命科学与技术学院， 呼和浩特 010022； 2. 内蒙古自治区高等学校生物多样性保护
    与可持续利用重点实验室， 呼和浩特 010022； 3. 内蒙古自治区农牧业技术推广中心， 呼和浩特 010010
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 作者简介:姜凯，博士，讲师，研究方向为环境微生物资源挖掘及利用，E-mail: jiangkai@imnu.edu.cn
  • 基金资助:
    内蒙古自然科学基金项目(2020BS03006)； 内蒙古师范大学引进高层次人才科研启动经费项目（2018YJRC015）

Bacteria diversity in Daihai Lake in Inner Mongolia

JIANG Kai1,2, CAO Chunling3   

  1. 1. College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China;
    2. Key Laboratory of Biodiversity Conservation and Sustainable Utilization for College and University of Inner
    Mongolia Autonomous Region, Hohhot 010022, China; 3. Agriculture and Animal Husbandry Technology
    Popularization Center of Inner Mongolia Autonomous Region, Hohhot 010010, China
  • Online:2023-04-18 Published:2023-04-18

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摘要： 采用培养方法和宏基因组测序技术分析内蒙古岱海湖水及沉积物中的细菌多样性。培养方法所分离细菌分属4门，6纲，18目，25科，47属，77种，其中，潜在新菌种20种，优势门为变形菌门（Proteobacteria）和厚壁菌门（Firmicutes）。培养过程中发现0.22 μm滤膜过滤处理能滤除大部分常见的细菌，提高新种检出效率。宏基因组测序共分析到细菌83个门，优势门为变形菌门和拟杆菌门（Bacteroidetes）。门水平还识别出一个未归类的细菌门和50个候选菌门，其中，33个为候选门级辐射类群（candidate phyla radiation, CPR）类群。结果表明，岱海中细菌具有较高的丰度和多样性，同时还存在丰富的CPR类群菌株。研究结果为下一步岱海微生物资源挖掘以及生态功能研究奠定基础。

关键词: 岱海, 细菌多样性, 宏基因组测序, 培养, 候选门级辐射类群

Abstract: Bacterial diversity in water and sediments of Daihai Lake was analyzed by culture method and metagenomic sequencing in this study. The bacteria isolated by culture method belonged to 4 phyla, 6 classes, 18 orders, 25 families, 47 genera, 77 species, including 20 potential novel species, the dominant phyla were Proteobacteria and Firmicutes. It was found that 0.22 μm filtration can remove most common bacteria and improve the detection of novel species. A total of 83 phyla were identified by metagenomic sequencing. Proteobacteria and Bacteroidetes were the dominant phyla. Metagenomic sequencing identified one unclassified phyla and 50 candidate phyla, of which 33 were candidate phyla radiation (CPR). The results showed that there are high abundance and diversity of bacteria, and abundant CPR strains in Daihai Lake. The results laid a foundation for further research on microbial resources mining and ecological functions in Daihai Lake.

Key words: Daihai Lake, bacterial diversity, metagenomic sequencing, culture, CPR

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