关键词: 实验教学, 原代培养, 以学为中心, 教学改革, 人才培养

Abstract: The experiment of animal-cell primary culture for undergraduates was reformed in order to solve the problems of students’ low success rate and insufficient training of abilities in traditional experimental teaching. Accordingly, a consolidated progressive experimental teaching mode was constructed and practiced. Aseptic technique and primary culture by enzymatic disaggregation, these important and widely applicated technologies, were practiced a lot. Primary culture by explantation and immunocytochemistry was phased to deepen the experiment teaching. Beyond that, students carried out the autonomous protocol designs, which were increasingly difficult. Extensibility, depth, comprehensiveness and design, therefore, were increased progressively with experiments. Students’ experiment results and survey results about the teaching effect showed that this consolidated progressive experimental teaching mode effectively enhanced their success rate. Their abilities, including practice, cooperation, analysis/solution of problems, exploration and innovation, were strengthened. It effectively solved the above issues in traditional experimental teaching and correspondingly improved the quality of experimental teaching.

Key words: experimental teaching, primary culture, learning-centered, teaching reform, talent cultivation