关键词: 通识教育, 微丝, 荧光标记技术, 实验教学改革

Abstract: The course group takes onion bulbs as the experimental materials to establish the experiment of observing of microfilaments by fluorescence labeling in the general elective course of the introduction to basic life science experiment. In this experiment, the microfilaments in the inner epidermis cells of onion are labeled by the phalloidin-rhodamine. Under the fluorescence microscope, the students can observe that the microfilaments in the onion inner epidermal cells emit red fluorescence. The microfilaments fill the whole cell and are interweaved with each other. Around the nucleus, the microfilaments are denser. According to the characteristics of general elective course, onion is used instead of animal cells as experimental material, which reduces the difficulty of experimental operation. The experiment helps the non-biological majors to enhance their understanding of the frontier knowledge and experimental technology of life science, and cultivate their interest in life science by interpreting the basic knowledge of cytoskeleton, microfilaments and fluorescent labeling technology and training the experimental method of labeling microfilaments withphalloidin-rhodamine. At the same time, it enriches the experimental contents, improves the teaching quality of the course and obtains good effects.

Key words: general education, microfilaments, fluorescent labeling technology, experimental teaching reform