生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 86-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 086

• 研究报告 • 上一篇    下一篇

旱、盐胁迫下棉花3个转录因子基因的表达与生理指标相关性分析

  

  1. 新疆农业大学农学院棉花教育部工程研究中心,乌鲁木齐830052
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 通讯作者: 陈全家,教授,研究方向为棉花分子育种,E-mail:chqjia@126.com
  • 作者简介:范　蓉,硕士研究生,研究方向为棉花分子育种,E-mail:1029681312@qq.com
  • 基金资助:
    转基因生物新品种培育重大专项项目(2016ZX08005-004-009)

Correlation nalysis of expression of three transcription factorgenesandphysiologicalindexes under cotton droughtandsaltstress

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  1. Cotton Engineering Research Center of Ministry of Education, College of Agriculture,Xinjiang Agricultural University, Urumqi 830052, China
  • Online:2021-08-18 Published:2021-08-18

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摘要： 土壤干旱及盐渍化对植物生长发育具有重要影响,筛选棉花抗旱性和耐盐性的基因资源十分重要。以2个抗摘旱耐盐要和2个敏旱敏盐棉花品种为材料,通过qRT-PCR检测3个转录因子GhHsfA7、GhbZIP15和GhNAC2在转录水平的表达差异,同时分析5个常用抗旱、耐盐性鉴定生理指标变化及这3个基因表达与生理指标间的相关性。结果表明,PEG胁迫6h及NaCl胁迫3h下,3个转录因子基因GhHsfA7、GhbZIP15和GhNAC2优势表达,且在敏感型棉花中的相对表达量显著低于耐受型品种。旱、盐胁迫下,棉花生理指标与对照之间存在显著或极显著差异,并且耐受型棉花品种中Pro、SOD及POD相对变化均大于敏感型品种,而MDA和EL相对变化均小于敏感型品种。相关性分析表明,GhHsfA7、GhbZIP15、GhNAC2与生理指标MDA、Pro、SOD、POD、EL间具有显著或极显著的相关性,因此可将转录因子GhHsfA7、GhbZIP15和GhNAC2的表达作为棉花抗旱耐盐性鉴定的分子指标,为棉花抗旱耐盐性研究奠定基础。

关键词: 棉花, PEG胁迫, NaCl胁迫, 转录因子, 生理指标, 相关性

Abstract: Soil drought and salinization have serious effects on plant growth and development. It is very important to screen genes suit-able for evaluating drought resistance and salt tolerance of cotton. Two drought-resistant and salt-tolerant cotton varieties and two drought-sensitive and salt-sensitive cotton varieties were used as materials to detect the expression difference of stress-resistant related transcrip-tion factorsGhHsfA7,GhbZIP15andGhNAC2at transcriptional level by qRT-PCR. The expression differences of stress-resistant related transcription factorsGhHsfA7,GhbZIP15andGhNAC2were detected at transcriptional level. At that same time, the changes of five com-mon physiological indexes for stress resistance identification, and the correlation between the expression of these three genes and physio-logical indexes were analyzed. The results showed that the relative expression levels ofGhHsfA7,GhbZIP15, andGhNAC2were predomi-nantly in sensitive cotton under 6 h PEG stress and 3 h NaCl stress, Which were significantly lower than those in the tolerant varieties.Under drought and salt stress, there were significant or extremely significant differences in physiological indexes between cotton and thecontrol, and the relative changes of Pro, SOD and POD in tolerant cotton varieties were greater than those in sensitive cotton varieties,while the relative changes of MDA and EL were smaller than those in sensitive cotton varieties. Correlation analysis showed thatGhHsfA7,GhbZIP15,GhNAC2had significant or extremely significant correlation with MDA, Pro, SOD, POD, and EL. Therefore, the expression of the transcription factorsGhHsfA7,GhbZIP15andGhNAC2can be used as molecular markers for the identification of drought resistanceand salt tolerance of cotton, which lays a foundation for the study of stress resistance mechanism of cotton.

Key words: cotton, PEG stress, NaCl stress, transcription factors, physiological index, correlation

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