生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (2): 54-.doi: 10.3969/j.issn.2095-1736.2026.02.054

• 研究报告 • 上一篇    下一篇

锌、铜复合胁迫对紫花苜蓿幼苗生长生理指标的影响

宋欣美1， 段士鑫1， 吴怀欣1， 李长印1， 贾春云2， 刘长风1   

  1. 1. 沈阳化工大学 环境与安全工程学院， 沈阳 110142； 2. 中国科学院沈阳应用生态研究所， 沈阳 110016
  • 出版日期:2026-04-18 发布日期:2026-04-23
  • 通讯作者: 贾春云，博士，副研究员，研究方向为重金属污染土壤修复，E-mail：jiachunyun@iae.ac.cn；刘长风，博士，教授，研究方向为土壤污染修复，E-mail：Changfeng.Liu@syuct.edu.cn；贾春云和刘长风为共同通信作者
  • 作者简介:宋欣美，硕士研究生，研究方向为土壤污染修复，E-mail：1107902259@qq.com
  • 基金资助:
    辽宁省科技计划联合计划（应用基础研究）项目（2023021253-JH2/1017）；辽宁省教育厅项目（JYTMS20231495）；沈阳市社会治理科技专项（24-213-3-10）

Effects of zinc and copper combined stress on growth and physiological indexes of alfalfa （Medicago sativa L.） seedlings

SONG Xinmei1, DUAN Shixin1, WU Huaixin1, LI Changyin1, JIA Chunyun2, LIU Changfeng1   

  1. 1. College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142,
    China; 2. Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
  • Online:2026-04-18 Published:2026-04-23

摘要： Zn、Cu作为饲料添加剂被广泛使用，因吸收率低，大量Zn、Cu随动物粪便排出体外并进入土壤，造成Zn、Cu污染。本研究采用紫花苜蓿种子为试验材料，探究Zn、Cu复合胁迫对紫花苜蓿幼苗各项生长生理指标的影响，以及紫花苜蓿幼苗对Zn、Cu的富集能力，并对各指标进行相关性分析和主成分分析，为后续紫花苜蓿修复土壤Zn、Cu污染提供依据。研究采用水培法，利用不同质量浓度Zn2+（0、100、200和500 mg/L）、Cu2+（0、50、100和200 mg/L）处理紫花苜蓿幼苗。结果表明：紫花苜蓿幼苗对Zn、Cu有一定的富集作用，但在复合胁迫下Zn的富集作用优于Cu。相关性分析表明，Zn、Cu复合胁迫时，幼苗体内的重金属含量、H2O2含量、MDA含量与复合胁迫浓度呈正相关，根细胞相对活性、叶绿素含量与复合胁迫浓度呈负相关。主成分分析表明，H2O2、脯氨酸、CAT可作为评价Zn、Cu复合胁迫对紫花苜蓿影响的主要指标。综合来看，紫花苜蓿可富集土壤中Zn、Cu，改善土壤生态环境。

关键词: 重金属胁迫, 植物耐受性, 生理响应, 相关性分析, 主成分分析

Abstract: Zinc （Zn） and copper （Cu） have been extensively used as feed additives. Owing to their low utilization efficiency by animals, substantial quantities of Zn and Cu were excreted in feces and subsequently entered the soil, leading to Zn and Cu pollution. This study used alfalfa （Medicago sativa L.） seeds as experimental material to examine the effects of combined Zn and Cu stress on various growth and physiological parameters of alfalfa seedlings, as well as their tolerance to Zn and Cu stress. Furthermore, correlation analysis and principal component analysis were conducted to provide a foundation for future research on the potential of alfalfa for remediating soil contaminated with Zn and Cu. The experiment was conducted using a hydroponic system, in which alfalfa seedlings were exposed to varying concentrations of Zn2+（0, 100, 200, 500 mg/L） and Cu2+（0, 50, 100, 200 mg/L） in a modified Hoagland solution. The findings indicated that the alfalfa seedlings accumulated Zn and Cu to some extent, with Zn accumulation being more pronounced under combined stress conditions. Correlation analysis demonstrated that, under the combined Zn and Cu stress, the concentrations of heavy metals, hydrogen peroxide （H2O2）, and malondialdehyde （MDA） in the seedlings were positively correlated with the stress concentration. Conversely, the stress concentration was negatively correlated with both relative root cell viability and chlorophyll content. Principal component analysis identified hydrogen peroxide （H2O2）, proline, and catalase （CAT） as pote

Key words: heavy metal stress, plant tolerance, physiological responses, correlation analysis, principal component analysis

中图分类号: