生物学杂志

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欧洲冬青对低温胁迫的生理响应及抗寒性评价

  

  1. 1. 南京林业大学 风景园林学院, 南京 210037;2. 中国科学院植物研究所 南京中山植物园, 南京 210014
  • 出版日期:2019-06-18 发布日期:2019-06-18
  • 通讯作者: 田如男,教授,研究方向为园林植物应用,E-mail:beike0607@aliyun.com
  • 作者简介:崔莺文,硕士,研究方向为园林植物应用,E-mail:542354928@qq.com
  • 基金资助:
    国家林业局“948”项目(2012-4-33);江苏高校“青蓝工程”资助项目;江苏高校品牌专业建设工程项目(PPZY2015A063)

Evaluation of cold resistance and physiological response to low temperature on five kinds of Ilex aquifolium

  1. 1. College of Landscape Architecture, Nanjing Forestry University,Nanjing 210037; 2. Nanjing Botanical Garden Mem. Sun Yat-sen, Institute of Botany, Chinese Academy of Sciences, Nanjing 210014, China
  • Online:2019-06-18 Published:2019-06-18

摘要: 以欧洲冬青(Ilex aquifolium)原种,2个欧洲冬青园艺杂交种[‘长叶阿尔塔’(Ilex×altaclerensis ‘Belgica Aurea’]、[‘金心阿尔塔’(Ilex×altaclerensis ‘Lawsoniana’)]和2个欧洲冬青品种[‘布利奥特夫人’(Ilex aquifolium. ‘Madame Briot’]、[‘小宝石’(Ilex aquifolium ‘Little Gem’)]的2年生扦插苗为试验材料,进行低温胁迫处理,观测形态特征,测定不同低温处理下的相对电导率(REC)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、可溶性糖含量和叶绿素总含量等生理指标,并用主成分分析法和隶属函数法对5种植物的抗寒性进行综合评价。结果表明,5种植物的抗寒性大小依次为:‘长叶阿尔塔’>‘小宝石’>欧洲冬青原种>‘布利奥特夫人’>‘金心阿尔塔’。

关键词: 欧洲冬青, 低温胁迫, 生理响应, 抗寒性, 综合评价

Abstract: In this paper,two years old seedlings of Ilex aquifolium,two hybrids of Ilex aquifolium (Ilex × altaclerensis‘Belgica Aurea’,Ilex × altaclerensis‘Lawsoniana’) and two varieties of Ilex aquifolium (Ilex aquifolium.‘Madame Briot’,Ilex aquifolium‘Little Gem’) were placed in different low temperature conditions, the morphological characteristics,relative conductivity,the activity of superoxide (SOD),the level of malondialdehyde (MDA),the contents of soluble sugar and chlorophyll were studied. Comprehensive evaluation of cold resistance on five kinds of plants was conducted by principal component analysis and subordinate function analysis. The results revealed that cold resistance of five kinds of plants from strong to weak was Ilex×altaclerensis ‘Belgica Aurea’>Ilex aquifolium ‘Little Gem’>Ilex aquifolium>Ilex aquifolium. ‘Madame Briot’>Ilex×altaclerensis ‘Lawsoniana’.

Key words: Ilex aquifolium, low-temperature stress, physiological response, cold resistance, comprehensive evaluation

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