生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 92-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 092

• 研究报告 • 上一篇    下一篇

圆锥南芥适应高山干旱环境机制

  

  1. 1.湖南科技大学生命科学学院经济作物遗传改良与综合利用湖南省重点实验室重金属污染土壤生态修复与安全利用湖南省高校重点实验室,湘潭411201; 2.中国科学院昆明植物研究所西南野生生物种质资源库,昆明650201
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 作者简介:唐　婷,博士,讲师,主要从事植物逆境生理研究,E-mail:tangting@ hnust.edu.cn
  • 基金资助:
    湖南省教育厅科学研究项目(18B215)92

Adaptation mechanism of Arabispaniculata to droughtstressinalpine environment

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  1. 1.School of Life Sciences, Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization,Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Hunan Universityof Science and Technology, Xiangtan 411201, China; 2. Plant Germplasm and Genomics Center, GermplasmBank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Online:2021-08-18 Published:2021-08-18

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摘要：

以高山植物圆锥南芥和其近缘物种拟南芥种子为研究材料,通过在生长培养基中添加不同浓度的甘露醇 (150、300和600 mmol / L) 模拟干旱胁迫,比较两物种的萌发情况,并检测甘露醇处理前后两种植物种子中的内源激素脱落酸(ABA) 含量、热激蛋白HSP101 和HSP70 的表达量,研究圆锥南芥适应高山干旱环境的可能机制。结果显示: 圆锥南芥种子对甘露醇更敏感,当甘露醇浓度超过300 mmol /L 时,出现暂时休眠的现象;正常情况下,圆锥南芥种子中ABA 含量显著高于拟南芥;甘露醇处理后,两物种ABA 含量都有所上升,圆锥南芥ABA 含量显著高于拟南芥;正常情况下,两物种的HSP101 和HSP70 表达量都较低;甘露醇处理后,拟南芥的HSP101 和HSP70 的表达量明显增强,而圆锥南芥的HSP 表达量不受影响。研究表明,圆锥南芥在干旱胁迫下保持高含量ABA 以启动种子休眠,推测其不合成HSP 蛋白等生物大分子物质是一种节约能量的方式,这是圆锥南芥对高山干旱环境的适应性策略。


关键词: 圆锥南芥, 种子萌发:种子休眠, 干旱胁迫, 内源激素ABA, 热激蛋白HSP

Abstract: The seeds ofArabispaniculataand its relativeArabidopsisthalianawere used as materials in the present study. The germi-nation rate, the content of ABA and the accumulation of HSP101 and HSP70 were compared in these two species before and after treat-mentofmannitolwithdifferentconcentrations(150,300and600mmol /L). TheadaptationmechanismofA.paniculatato alpine en-vironment was discussed. The results were presented as following: the seeds ofA.paniculatawere more sensitive to the treatment of mannitol; temporary seed dormancy was exhibited inA.paniculatawhen the concentration of mannitol exceeded 300 mmol / L; the ABA content of the seeds inA.paniculatawas significantly higher than that inA.thalianaunder the normal growth conditions and mannitol treatment. The abundance of HSP101 and HSP70 was low in both species under normal growth conditions. The protein levels of HSP101 and HSP70 were increased rapidly under mannitol treatment inA.thaliana, while those were still kept low inA.paniculata.These results indicated that seed dormancy was induced inA.paniculataupon high accumulation of ABA to adaptation to alpine envi-ronment with limited water in winter. A energy conservation strategy was speculated according to the fact that the protein levels ofHSP101 and HSP70 were not affected inA.paniculataunder drought stress.K

Key words: Arabispaniculata, seed germination, seed dormancy, drought, ABA, HSP

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