生物学杂志 ›› 2021, Vol. 38 ›› Issue (4): 82-.doi: 10. 3969 / j. issn. 2095-1736. 2021. 04. 082

• 研究报告 • 上一篇    下一篇

Bt蛋白在晋南地区转基因棉田中的残留分析

  

  1. 1.运城学院生命科学系,运城044000; 2.运城学院理科实验中心,运城044000
  • 出版日期:2021-08-18 发布日期:2021-08-18
  • 作者简介:关正君,博士,教授,从事生态安全评价研究,E-mail:zhengjunguan@126.com
  • 基金资助:
    山西省自然科学基金项目(201701D121089);山西省“1331”工程重点学科项目(098-091704);运城学院“特色农产品发展”学科群学科项目(XK-2019001);运城学院博士科研启动项目(YQ-2014022)

Residue analysis of Btproteinintransgeni ccotton field #br#

  1. 1. Department of Life Science, Yuncheng University, Yuncheng 044000, China; 2. Experimental Centre for Science, Yuncheng University, Yuncheng 044000, China
  • Online:2021-08-18 Published:2021-08-18

摘要: 随着抗虫转Bt基因棉花长期大面积推广种植,Bt蛋白通过植株器官残体、根系分泌物等方式进入土壤。土摘壤中要Bt蛋白的残留和积累是否会给农田生态系统带来危害,逐渐成为公众关注的焦点。研究采用ELISA检测法对晋南地区5个区域的转Bt基因棉田中根际土Bt蛋白残留情况和转基因棉花根、叶Bt蛋白表达含量进行分析。结果表明,各转基因棉田样地根际土与非根际土的Bt蛋白含量均存在显著差异。转基因棉花根际土中Bt蛋白含量最高为0.184 8 ng /g,最低为0.1120 ng /g。表明连续种植转基因棉花的棉田土壤中可能有低剂量的Bt蛋白残留。晋南地区5个区域中转基因棉花的根与叶之间Bt蛋白含量均有显著差异。转基因棉花根Bt蛋白含量最高为19.34ng /g,最低为12.57 ng /g;叶Bt蛋白含量最高为128.11 ng /g,最低为83.19 ng /g。表明晋南地区大田种植的转基因棉花在其收获期棉叶Bt蛋白含量显著高于根系Bt蛋白含量。研究结果为转基因棉花合理的商业化种植和转Bt基因作物的生态安全评价提供一定的参考依据。

关键词: 转基因棉花, Bt蛋白, 残留分析, ELISA

Abstract: With the long-term large-scale cultivation of transgenicBtcotton, Bt proteins were introduced into the soil by means of plant organ residues and root exudates. Whether the residue and accumulation of Bt protein in soil will bring harm to farmland ecosystem hasgradually become the focus of public attention. In this paper, the residue of Bt protein in rhizosphere soil and the expression of Bt pro-tein in transgenic cotton roots and leaves in five areas of southern Shanxi province were analyzed by ELISA technology. The results showed that there were significant differences in Bt protein content between rhizosphere soil and non-rhizosphere soil in the five investi-gated areas. The highest Bt protein content in rhizosphere soil of transgenic cotton was 0. 184 8 ng / g, and the lowest was 0. 112 0 ng / g.It was suggested that there might be a low dose of Bt protein residues in the soil after transgenic Bt cottons were continuously planted.Furthermore, there were significant differences in Bt protein content between roots and leaves of transgenic cotton in the five regions ofsouthern Shanxi province. The highest Bt protein content in transgenic cotton roots was 19. 34 ng / g and the lowest was 12. 57 ng / g, while the highest Bt protein content in leaf was 128. 11 ng / g and the lowest was 83. 19 ng / g. The results showed that Bt protein content of transgenic cotton leaves was higher than that of roots in all sampling regions. This experiment research results will provide scientificbasis for reasonable cultivation of transgenic cottons and ecological safety evaluation of Bt crop

Key words: transgenic cotton, Bt protein, residue analysis, ELISA

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