生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (4): 1-.doi: 10.3969/j.issn.2095-1736.2024.04.001

• 研究报告 •    下一篇

AsmiR156：沙蓬干旱记忆分子调控网络的潜在枢纽

房庭舟， AWUKU Ibrahim， 燕　霞， 钱朝菊， 范兴科， 赵鹏姝， 廖钰秋， 史　梁， 马小飞   

  1. 中国科学院西北生态环境资源研究院， 兰州 730030
  • 出版日期:2024-08-18 发布日期:2024-08-14
  • 通讯作者: 马小飞，博士，研究员，研究方向为荒漠资源植物的开发与大健康应用，E-mail:maxiaofei@lzb.ac.cn
  • 作者简介:房庭舟，博士，研究方向为荒漠植物抗逆的分子机理，E-mail:565321232@qq.com
  • 基金资助:
    国家自然科学基金项目（31901079, 32271695, 32201378, 32171608）；中国科学院战略生物资源计划（KFJ-BRP-007-015）

AsmiR156: potential hub in drought memory molecular regulatory network of Agriophyllum squarrosum (L.) Moq. #br#

FANG Tingzhou, AWUKU Ibrahim, YAN Xia, QIAN Chaoju, FAN Xingke, ZHAO Pengshu,LIAO Yuqiu, SHI Liang, MA Xiaofei   

  1. Northwest Institute of Ecology & Environment Resources, CAS, Lanzhou 730030, China
  • Online:2024-08-18 Published:2024-08-14

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摘要： 为探索miRNA-mRNA如何参与沙蓬干旱响应和干旱记忆，选择沙蓬干旱敏感生态型AEX为植物材料，开展3轮干旱-复水循环实验后进行转录组和sRNA测序。分别鉴定出341个和7858个响应干旱胁迫的沙蓬miRNA（DEMIs）和mRNA（DEMs）。DEMI-DEM调控网络构建表明，24个沙蓬DEMIs参与调控1423个DEMs，这些靶基因主要参与细胞分化功能。鉴定出两个干旱记忆miRNA（DMMIs） miR156和miR6173，分别调控11个和6个干旱记忆基因（DMGs）。miR156的靶基因包括3个转录因子（TF）AsRWP-RK、AsC3H11和AsWRKY33；miR6173的靶基因包括两个GST（谷胱甘肽S转移酶）。在先前的研究中，AsC3H11和AsWRKY33作为TF分子开关参与沙蓬干旱记忆分级调控网络，因此，沙蓬miR156可作为“miRNA-转录因子-功能基因”网络的候选枢纽。研究解析了沙蓬干旱响应和干旱记忆的分子调控基础，将为荒漠植物中的抗逆遗传资源开发提供理论依据。

关键词: 沙蓬, 干旱胁迫, 干旱记忆, miRNA, 转录组

Abstract: In order to detect how miRNA-mRNA interaction participates in drought response and drought memory ofA. squarrosum, the drought-sensitive ecotype AEX ofAgriophyllum squarrosumwas selected as the plant material, and transcriptome and sRNA sequencing were performed after three rounds of drought-rehydration treatments. A total of 341 and 7858A. squarrosummiRNAs (DEMIs) and mRNAs (DEMs) were identified in response to drought stress, respectively. The DEMI-DEM regulatory network showed that 24A. squarrosumDEMIs were involved in the regulation of 1423 DEMs, and these target genes were mainly involved in cell differentiation function. Two drought memory miRNAs (DMMIs), miR156 and miR6173, were identified, which regulated 11 and 6 drought memory genes (DMGs), respectively. Target genes for miR156 included three transcription factors (TFs):AsRWP-RK,AsC3H11, andAsWRKY33; the target genes for miR6173 included twoGSTs (glutathione S-transferase). In previous study,AsC3H11andAsWRKY33participated in theA. squarrosumdrought memory hierarchical regulatory network as TF molecular switches, soA. squarrosummiR156 could serve as a candidate hub for the “miRNA-transcription factor-functional gene” network. This study partially elucidated the molecular regulatory basis of drought response and drought memory inA. squarrosum, which will provide a theoretical basis for the development of stress-tolerant genetic resources in desert plants.

Key words: A. squarrosum, drought stress, drought memory, miRNA, transcriptome

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