生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 71-.doi: 10.3969/j.issn.2095-1736.2023.02.071

• 研究报告 • 上一篇    下一篇

干旱胁迫下锌对马铃薯组培苗生长及生理指标的影响#br#

党伟英1， 唐　勋1,2， 赵　婧3， 赵贵宾4， 朱永永4， 张　宁1,2， 司怀军1,2   

  1. 1. 甘肃农业大学 生命科学技术学院， 兰州 730070； 2. 甘肃省干旱生境作物学省部共建
    国家重点实验室， 兰州 730070； 3. 甘肃省农业信息中心， 兰州 730030;
    4. 甘肃省农业技术推广总站， 兰州 730020
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 通讯作者: 司怀军，博士，教授，主要从事植物生物技术教学和研究工作，E-mail: hjsi@gsau.edu.cn
  • 作者简介:党伟英，硕士研究生，主要研究方向为马铃薯锌转运机制，E-mail: 1477833678@qq.com
  • 基金资助:
    甘肃富锌马铃薯生产技术集成与应用项目（GNKJ-2021-25）； 甘肃省马铃薯绿色高质高效创建综合配套技术集成与研究应用项目（GNKJ-2021-26）； 联合国世界粮食计划署（WFP）项目（WFPGSPP-1）

Effects of zinc on growth and physiological indices of potato plantlets under drought stress #br#

DANG Weiying1, TANG Xun1,2, ZHAO Jing3, ZHAO Guibin4, ZHU Yongyong4,ZHANG Ning1,2, SI Huaijun1,2   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2. State
    Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; 3. Gansu
    Agricultural Information Center, Lanzhou 730030, China; 4. Gansu General Station of Agro-technology Extension,
    Lanzhou 730020, China
  • Online:2023-04-18 Published:2023-04-18

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摘要： 为阐明干旱胁迫下锌对马铃薯生长发育的影响，以马铃薯栽培品种‘大西洋’组培苗为试验材料，用不同锌含量（0、8.6、17.2、25.8、34.4和43 mg/L）的培养基培养15 d，再置换为含有20% PEG6000的培养基模拟干旱胁迫继续培养15 d，测定组培苗的表型、抗氧化酶活性以及渗透调节物质含量，并对抗旱性进行分析。结果发现：锌对马铃薯组培苗在PEG胁迫下的生长有显著影响，组培苗在培养基锌含量为17.2 mg/L时均表现最佳。其中，鲜重和株高在培养基锌含量为17.2 mg/L时较正常MS处理分别增长了50%和34.66%；叶片的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和Pro含量较正常MS处理分别增长了9.88%、10%和3.88%，MDA含量较正常MS处理下降了21.86%。结果表明外源施加适量锌可以促进组培苗的生长发育，培养基锌含量为17.2 mg/L时能够显著提高马铃薯组培苗的耐旱性。研究结果为进一步阐明锌调控马铃薯抗旱机制提供了基础。

关键词: 马铃薯, 锌, 干旱胁迫, 抗氧化酶活性, 表型

Abstract: In order to elucidate the effect of zinc on the growth and development of potato under drought stress, the tissue culture seedlings of potato cultivar “Atlantic” , and the medium with different zinc contents (0, 8.6, 17.2, 25.8, 34.4 and 43 mg/L) were used as the test material. After cultured for 15 d, the medium was replaced with medium containing 20% PEG6000 to simulate drought stress for 15 d. The phenotype, antioxidant enzyme activity and osmotic adjustment substance content of the tissue culture seedlings were determined, and the drought resistance was analyzed. The results showed that zinc had a significant effect on the growth of potato tissue culture seedlings under PEG stress. The tissue culture seedlings performed best when the zinc content of the medium was 17.2 mg/L. Among them, the fresh weight and plant height increased by 50% and 34.66% respectively when the zinc content of the medium was 17.2 mg/L compared with that of the normal MS treatment; the activities of catalase (CAT), superoxide dismutase (SOD) and Pro Compared with that of the normal MS treatment, the content increased by 9.88%, 10% and 3.88% respectively, and the MDA content decreased by 21.86% compared with that of the normal MS treatment. The results showed that exogenous application of an appropriate amount of zinc could promote the growth and development of tissue culture seedlings, and when the zinc content of the medium was 17.2 mg/L, the drought tolerance of potato tissue culture seedlings could be significantly improved. The results provided a basis for further elucidating the mechanism of zinc regulation of potato drought resistance.

Key words: potato, zinc, drought stress, antioxidant enzyme activity, phenotype

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