生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 27-.doi: 10.3969/j.issn.2095-1736.2023.04.027

• 研究报告 • 上一篇    下一篇

TAP酞菁锌光敏剂对大肠杆菌的抗菌活性

鄢树枫1， 廖晓云1， 师　琪1， 吴汉钦1， 黄晓晨2   

  1. 1. 三明学院 资源与化工学院， 三明 365004； 2. 中国科学院 福建物质结构研究所， 福州 350002
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 作者简介:鄢树枫，博士，副教授，研究方向为生物医药研究，E-mail:ysfready@163.com
  • 基金资助:
    福建省自然科学基金青创项目（2020J05092）； 福建省中青年教师教育科研项目(JAT190705;B201918)； 三明学院国家基金培育项目(PYT2004)

Antibacterial activity of tap zinc phthalocyanine photosensitizer against Escherichia coli

YAN Shufeng1, LIAO Xiaoyun1, SHI Qi1, WU Hanqin1, HUANG Xiaochen2   

  1. 1. School of Resource and Chemical Engineering, Sanming University, Sanming 365004, China; 2. Fujian
    Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 为探究TAP\[2,4,6-三（二甲氨甲基）苯酚）\]酞菁锌光敏剂对大肠杆菌的抗菌活性，通过紫外OD检测法测定大肠杆菌的生长曲线图，确定大肠杆菌的最适生长期；利用紫外光谱法测定获得TAP酞菁光敏剂的紫外-浓度标准曲线；采用稀释涂板法、试剂盒检测法（CCK8）和探针法（DCFH-DA）等研究TAP酞菁锌光敏剂对大肠杆菌的抗菌作用。试验数据表明：大肠杆菌的对数生长期约在接种后4～10 h；TAP酞菁锌光敏剂对大肠杆菌具有显著的光动力抗菌活性，其抗菌作用与光敏剂浓度呈正比；TAP酞菁锌光敏剂可能是通过产生氢氧自由基型单线态氧发挥抗菌作用。研究证明TAP酞菁锌光敏剂对大肠杆菌有强光动力抗菌作用，为TAP酞菁锌光敏剂及其系列抗菌产品开发和利用提供参考。

关键词: TAP酞菁锌, 光敏剂, 大肠杆菌, 单线态氧, 抗菌活性

Abstract: To study the antibacterial activity and mechanism of tap (2,4,6-tris (Dimethylaminomethyl) phenol) zinc phthalocyanine photosensitizer against Escherichia coli (E.coli, ATCC 25922), the growth curve of E.coli was determined by UV, and the antibacterial activity of TAP zinc phthalocyanine photosensitizer on E.coli was studied by agar plate method. The metabolic activity was measured via the CCK-8 assay. The mode of action of tap zinc phthalocyanine photosensitizer was analyzed by ROS probe method (Quantifying ROS levels using DCFH-DA probe). Results showed that the logarithmic growth period of E.coli was about 4-10 h after inoculation. TAP zinc phthalocyanine photosensitizer had significant photodynamic antibacterial activity against E.coli, and its antibacterial effect was directly proportional to the concentration of photosensitizer. At the same time, it was proved that TAP zinc phthalocyanine photosensitizer played an antibacterial role by producing hydroxyl free radical singlet oxygen. This research proved that TAP zinc phthalocyanine photosensitizer had strong photodynamic antibacterial effect on E.coli, which provided evidence for the development-series of antibacterial products based on TAP zinc phthalocyanine photosensitize for antibacterial purpose.

Key words: TAP Zinc phthalocyanine, photosensitizer, E.coli, singlet oxygen, antibacterial activity

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