生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 20-.doi: 10.3969/j.issn.2095-1736.2023.04.020

• 研究报告 • 上一篇    下一篇

发酵优化绿针假单胞菌GP72-ND3ΔphzO高产吩嗪-1-羧酸

刘桢桢， 王　威， 黄显清， 张雪洪   

  1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室， 上海 201100
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 通讯作者: 王威，博士，副教授，研究方向为微生物代谢工程与合成生物学、生物物质的分离纯化、农业生物药物等，E-mail:weiwang100@sjtu.edu.cn
  • 作者简介:刘桢桢，硕士研究生，研究方向为微生物发酵，E-mail:haibara-4869@sjtu.edu.cn
  • 基金资助:
    国家重点研发计划项目（2019YFA0904302）

Enhancement of phenazine-1-carboxylic acid production for Pseudomonas chlororaphis GP72-ND3ΔphzO by fermentation optimization

LIU Zhenzhen, WANG Wei, HUANG Xianqing, ZHANG Xuehong   

  1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology,
    Shanghai Jiao Tong University, Shanghai 201100, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 对绿针假单胞菌GP72-ND3ΔphzO的发酵条件进行优化并在5 L反应器中进行放大培养。在摇瓶水平通过单因素实验、正交实验、最陡爬坡实验和中心组合设计实验，优化得到最佳培养基组成为甘油49.55 g/L、大豆蛋白胨37.34 g/L、KCl 1.5 g/L、MgSO40.75 g/L、K2HPO40.225 g/L，发酵60 h后吩嗪-1-羧酸（phenazine-1-carboxylic acid，PCA）的最大产量为（6.01±0.17） g/L，是优化前的2.6倍。在1 L反应器中优化发酵pH，当培养过程pH值维持在6.8时，PCA的产量最高，为（7.16±0.04） g/L，是优化前的1.2倍。在最优培养基和pH条件下，对菌株在5 L反应器中进行放大培养，发酵60 h后PCA的最大产量为（6.84±0.53） g/L。研究为提高PCA的生物合成速率和工业化应用提供支持。

关键词: 绿针假单胞菌GP72-ND3ΔphzO, 吩嗪-1-羧酸, 发酵优化, 中心组合设计, 反应器

Abstract: The fermentation conditions of this strain were optimized and scaled up in a 5 L bioreactor. First, through single factor experiment, orthogonal experiment, steepest climbing experiment and central composite design experiment at the shake flask level, the optimal medium composition was obtained as 49.55 g/L glycerol, 37.34 g/L soy peptone, 1.5 g/L KCl, 0.75 g/L MgSO4and 0.225 g/L K2HPO4, and the maximum production of phenazine-1-carboxylic acid (PCA) after 60 h fermentation was （6.01±0.17） g/L, which was 2.6 times as that before optimization. Then, the fermentation pH was optimized in 1 L bioreactors. When the pH was maintained at 6.8 during the culture process, the PCA production was the highest at （7.16±0.04） g/L, which was 1.2 times as that before optimization. Finally, under the optimal medium and pH conditions, the strain was scaled up cultured in 5 L bioreactors, and the maximum PCA production after 60 h of fermentation was (6.84±0.53) g/L. This study provided support for improving the production and industrial application of PCA.

Key words: Pseudomonas chlororaphis GP72-ND3ΔphzO, phenazine-1-carboxylic acid, fermentation optimization, central composite design, bioreactor

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