生物学杂志 ›› 2024, Vol. 41 ›› Issue (1): 32-.doi: 10.3969/j.issn.2095-1736.2024.01.032

• 研究报告 • 上一篇    下一篇

基于转录组测序的兔儿伞羽扇豆醇合成途径及关键酶基因研究

张京晶1,2, 许景垚1,2, 单婷玉1,2, 赵历强1,2, 钟欣欣1,2, 张帅帅1,2, 吴家文2,3,4   

  1. 1. 安徽中医药大学 研究生院, 合肥 230012; 2. 安徽中医药大学 科研技术中心 
    新安医学教育部重点实验室, 合肥 230038; 3. 安徽省中医药科学院, 合肥 230012;
    4. 安徽道地中药材品质提升协同创新中心, 合肥 230012
  • 出版日期:2024-02-18 发布日期:2024-02-18
  • 通讯作者: 吴家文,教授,研究方向为中草药基因和转录组学研究,E-mail:wujiawen@ahtcm.edu.cn
  • 作者简介:张京晶,硕士研究生,主要从事中药材转录组学研究,E-mail:Zhangjingjing@stu.ahtcm.edu.cn
  • 基金资助:
    国家重点研发计划项目(2017YFC1701600);安徽省自然科学基金项目(2008085MH268);安徽省高等学校科学研究项目(2022AH050474)

Identification of key enzyme genes involved in lupeol synthesis pathway in Syneilesis aconitifolia by transcriptome analysis

ZHANG Jingjing1,2, XU Jingyao1,2, SHAN Tingyu1,2, ZHAO Liqiang1,2, ZHONG Xinxin1,2,ZHANG Shuaishuai1,2, WU Jiawen2,3,4   

  1. 1. Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China; 2. Key Laboratory of Xin’an
    Medicine, Ministry of Education, Experimental Center for Scientific Research, Anhui University of Chinese Medicine,
    Hefei 230038, China; 3. Anhui Academy of Chinese Medicine, Hefei 230012, China; 4. Synergetic Innovation
    Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei 230012, China
  • Online:2024-02-18 Published:2024-02-18

摘要: 为解析兔儿伞羽扇豆醇生物合成途径,探究其关键酶基因,研究运用DNBSEQ测序平台对兔儿伞的叶、茎、根及根茎进行转录组测序,从头组装后获得了191541条Unigenes。KEGG代谢通路分析表明有961条Unigenes涉及兔儿伞羽扇豆醇生物合成途径,其中,395条Unigenes编码羽扇豆醇生物合成途径的17个关键酶。根与其他各组织比较,24条共有差异表达基因涉及羽扇豆醇生物合成途径,编码了法尼基焦磷酸合酶(farnesyl diphosphate synthase,FPPS)、角鲨烯合成酶(squalene synthase,SS)、角鲨烯环氧酶(squalene epoxidase,SE)等关键酶。对关键酶FPPS、SS和SE进行结构分析,发现它们均具有保守的催化结构域和底物结合结构域。研究丰富了兔儿伞植物的功能基因数据库,为进一步研究羽扇豆醇生物合成途径及其关键酶基因的功能和调控机制奠定了基础。

关键词: 兔儿伞, 转录组测序, 羽扇豆醇, 法尼基焦磷酸合酶, 角鲨烯合成酶, 角鲨烯环氧酶

Abstract: order to analyze the lupeol biosynthetic pathway in Syneilesis aconitifolia and explore its key enzyme genes, DNBSEQ sequencing platform was used to sequence the transcriptome of leaves, stems, roots and rhizomes of Syneilesis aconitifolia, and 191 541 Unigenes were obtained after de novo assembly. KEGG metabolic pathway analysis showed that 961 Unigenes were involved in the lupeol biosynthetic pathway in Syneilesis aconitifolia, of which 395 Unigenes encoded 17 key enzymes of the biosynthetic pathway. Comparing root with other tissues, 24 shared differentially expressed genes were involved in the lupeol biosynthetic pathway, encoding farnesyl diphosphate synthase (FPPS), squalene synthase (SS), squalene epoxidase (SE) and other key enzymes. Structural analysis of the key enzymes FPPS, SS and SE, showed that they all had conserved catalytic domains and substrate binding domains. This work enriched the functional gene database of Syneilesis aconitifolia, and laid a foundation for further study of the lupeol biosynthetic pathway and the function and regulation mechanism of key enzyme genes.

Key words: Syneilesis aconitifolia, transcriptome sequencing, lupeol, farnesyl diphosphate synthase, squalene synthase, squalene epoxidase

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