生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (1): 123-.doi: 10.3969/j.issn.2095-1736.2024.01.123

• 教学研究 • 上一篇    下一篇

一种适用于实验教学的果蝇DNA提取新方法

魏　远， 王宏刚， 朱玉山， 李艳君   

  1. 南开大学 生物国家级实验教学示范中心， 天津 300071
  • 出版日期:2024-02-18 发布日期:2024-02-18
  • 通讯作者: 朱玉山，博士，教授，研究方向为细胞自噬与细胞凋亡调控，E-mail:zhuys@nankai.edu.cn
  • 作者简介:魏远，硕士，实验师，主要研究方向为实验教学和实验室管理，E-mail:weiyuan@nankai.edu.cn
  • 基金资助:
    2020年天津市高等学校本科教学质量与教学改革研究计划项目(B201005506)

A new method to extract DNA from Drosophila melanogaster suitable for experimental teaching #br#

WEI Yuan, WANG Honggang, ZHU Yushan, LI Yanjun   

  1. National Experimental Teaching Demonstration Center of Biology, Nankai University, Tianjin 300071, China
  • Online:2024-02-18 Published:2024-02-18

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摘要： 南开大学遗传学实验课程组将科研中常用的动物组织DNA提取方法改造后用于实验教学，获得了1种适用于实验教学的果蝇DNA提取新方法。该方法由碱裂解、酸中和、离心3个步骤组成，具有操作简单、时间短、成本低、效果稳定等特点，获得的DNA无论是浓度还是纯度均可满足后续PCR扩增的需要，符合实验教学的要求。该方法简化了果蝇DNA的提取流程，为与果蝇基因表达分析相关实验项目的改革奠定了基础，有助于提升实验教学效果，推动课程建设。

关键词: 果蝇, DNA提取, 碱裂解法, 遗传学实验, 实验教学

Abstract: The common animal tissue DNA extraction method has been modified in the genetics course at Nankai University. This novel method of extracting DNA from Drosophila melanogaster can be applied in scientific research as well as in experimental teaching scenarios. The method comprises three steps which are namely alkali cracking, acid neutralization, and centrifugation. The method is characteristically simple, quick, stable, and cost-effective. The concentration and purity of the extracted DNA enable subsequent PCR amplification and are also conducive to experimental teaching. This method has simplified the process of extracting DNA from Drosophila melanogaster and has laid a foundation for the improvement of experimental projects related to Drosophila melanogaster gene expression analysis. In addition, it has helped to improve the experimental teaching effect and curriculum design.

Key words: Drosophila melanogaster, DNA extraction, alkaline lysis, genetic experiment, experimental teaching

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