生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (5): 117-.doi: 10.3969/j.issn.2095-1736.2025.05.117

• 教学研究 • 上一篇    下一篇

结果动态可视的细胞基因工程实验教学探索与实践

邱菊辉1,2,3， 蒲　颖1,2,3， 雷明星1,2， 吴　伟1,2,3， 王业启1,2， 张筱娟1,2,3，王贵学1,2,3， 罗彦凤1,2   

  1. 1. 重庆大学 生物工程学院， 重庆 400030； 2. 生物流变科学与技术教育部重点实验室，
    重庆 400030； 3. 血管植入物开发国家地方联合工程实验室， 重庆 400030
  • 出版日期:2025-10-18 发布日期:2025-10-14
  • 通讯作者: 王贵学，博士，教授，研究方向为生物医学工程与细胞基因工程，E-mail：wanggx@cqu.edu.cn；罗彦凤，博士，教授，研究方向为组织工程与细胞基因工程，E-mail：yfluo@cqu.edu.cn；王贵学和罗彦凤为共同通信作者
  • 作者简介:邱菊辉，博士，研究员，研究方向为生物工程教学、斑马鱼研究，E-mail：jhqiu@cqu.edu.cn
  • 基金资助:
    重庆大学实验教学改革项目（2021S40）； 重庆市高等教育教学改革研究重大项目（171003）； 重庆大学生物工程一流专业核心课程群（2023）； 国家自然科学基金青年科学基金项目（31701275）

Teaching exploration and practice on cellular and genetic engineering experiments featuring dynamically visualized results

QIU Juhui1,2,3, PU Ying1,2,3, LEI Mingxing1,2，WU Wei1,2,3, WANG Yeqi1,2,ZHANG Xiaojuan1,2,3, WANG Guixue1,2,3, LUO Yanfeng1,2   

  1. 1. College of Bioengineering, Chongqing University, Chongqing 400030, China; 2. Key Laboratory for Biorheological
    Science and Technology （Chongqing University）, Ministry of Education, Chongqing 400030, China;
    3. State and Local Joint Engineering Laboratory for Vascular Implants, Chongqing 400030, China
  • Online:2025-10-18 Published:2025-10-14

摘要： 为激发学生兴趣、培养适应产业发展需求的专业人才，重庆大学细胞基因工程实验课程团队基于转基因斑马鱼开展细胞基因工程研究，构建了直观可视化、病理现象化、动态实时化和手动操作化的转基因斑马鱼实验体系，首先构建表达硝基还原酶（NTR）和荧光蛋白的转基因鱼，然后用甲硝唑（MTZ）处理杀死荧光阳性细胞，直观可视地观察到转基因鱼脑部血管出血的病理现象。该实验体系包含细胞基因工程课程的所有核心实验，将该科研成果运用于课程教学，有效提高了本科课程的高阶性和创新性，也为本研一体化的细胞基因工程教学提供了新思路，提高了培养质量和培养效率。该实验课程的改革实践将为生物工程国家级一流本科专业的建设和生物医学工程一流学科建设赋能，为发展生物工程国家级一流本科专业注入动力。

关键词: 细胞基因工程实验教学, 结果动态可视, 斑马鱼, 本研一体化, 教学探索

Abstract: To cultivate students’ interest and develop professionals who can adapt to industry developments, building on our team’s research in transgenic zebrafish genetic engineering, this course introduced an experimental system that features visual intuition, pathological phenomena, dynamic real-time observation, and hands-on operations. The transgenic fish expressing nitroreductase (NTR) and fluorescent proteins based on the metronidazole/nitroreductase (MTZ/NTR) cell ablation system was constructed. Treatment with MTZ eliminated fluorescent-positive cells, and pathological manifestations including brain hemorrhage, thereby generating visually intuitive results. This experiment covered all core experiments in the cell genetic engineering course. Applying these research findings to course teaching enhanced the advanced and innovative nature of undergraduate courses and provide new approaches for integrated undergraduate-graduate genetic engineering education, improving the quality and efficiency of training. This addressed the demands for experimental teaching innovation and practice. This experimental reform practice empowered the construction of double first-class disciplines, and the development of national first-class undergraduate disciplines in biomedical engineering and bioengineering, thereby advancing national first-class undergraduate programs in bioengineering.

Key words: experiment teaching of cellular genetic engineering, dynamic visualization of results, zebrafish, undergraduate and graduate integration course, teaching exploration

中图分类号: