生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (5): 53-.doi: 10.3969/j.issn. 2095-1736.2021.05.053

• 研究报告 • 上一篇    下一篇

铁皮石斛DoTIFY基因家族全基因组鉴定及在原球茎发育过程中的表达

  

  1. 西南交通大学　生命科学与工程学院，成都610031
  • 出版日期:2021-10-18 发布日期:2021-10-21
  • 通讯作者: 王万军，教授，主要从事植物发育生物学研究，E-mail: wanjunwang@home.swjtu.edu.cn
  • 作者简介:胡　睿，硕士研究生，主要从事植物发育生物学研究，E-mail: 1143611129@qq.com
  • 基金资助:
    国家自然科学基金项目 (31371232)； 2017年度理工类科技创新项目(A0920502051722-88)

Genome-wide identification and analysis of the TIFY gene family in Dendrobium officinale Kimura et Migo during protocorm development

  1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
  • Online:2021-10-18 Published:2021-10-21

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摘要： 在铁皮石斛基因组数据中共鉴定出20个铁皮石斛TIFY(DoTIFY)基因。根据铁皮石斛、拟南芥、水稻、向日葵、陆地棉中TIFY的系统发育分析，将DoTFY分为4类：JAZ、PPD、ZIM和TIFY。铁皮石斛原球茎发育5个时期的转录组分析表明，有11个DoTIFY基因有差异性表达，表明这些基因可能参与调控铁皮石斛原球茎的发育。进一步对其中4个DoTIFY基因qPCR分析，DoJAZ3、8、5和DoJAZ6的表达水平在铁皮石斛原球茎生长发育的P1~P7时期显著增加，表明它们可能在P1~P7时期中起作用。研究结果为进一步研究铁皮石斛原球茎发育调控机制和相关的育种改良提供重要参考。

关键词: 铁皮石斛, TIFY, 生物信息学分析, RNA-seq, qPCR

Abstract: The identification of TIFYs in Dendrobium officinale is not particularly clear. In this study, 20 D. officinale TIFY(DoTIFY) genes were identified. According to the phylogenetic analysis of TIFYs from D. officinale, Arabidopsis thaliana, Oryza sativa, Gossypium arboretum and Helianthus annuus, DoTIFYs were divided into four categories, JAZ, PPD, ZIM, and TIFY. RNA-seq analysis during the 5 stages of D. officinale protocorm development revealed 11 differentially expressed DoTIFY genes and these genes might be involved in the development of D. officinale protocorm. The qPCR analysis showed that the expression levels of DoJAZ3, 8, 5, and DoJAZ6 increased significantly during P1-P7 stages of D. officinale, indicating that they might play roles in P1-P7 stages. The result help us for the further research on the mechanism of protocorm development and related breeding improvement of D. officinale.

Key words: Dendrobium officinal Kimura et Migo, TIFY, bioinformatics analysis, RNA-seq, qPCR

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