生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (3): 39-.doi: 10.3969/j.issn.2095-1736.2026.03.039

• 研究报告 • 上一篇    下一篇

基于组学数据鉴定马铃薯低温胁迫块茎休眠解除相关基因

杜炫蓉1,2， 张　宁1,2， 司怀军1,2   

  1. 1. 甘肃农业大学 生命科学技术学院， 兰州 730070；
    2. 甘肃农业大学 干旱生境作物学国家重点实验室， 兰州 730070
  • 出版日期:2026-06-18 发布日期:2026-06-16
  • 通讯作者: 司怀军，博士，教授，研究方向为马铃薯生物技术育种和分子生物学，E-mail：hjsi@gsau.edu.cn
  • 作者简介:杜炫蓉，硕士研究生，研究方向为生物技术育种，E-mail：dxr000818@163.com
  • 基金资助:
    甘肃省种业攻关项目（ZYGG-2025-7）

Identification of genes associated with potato (Solanum tuberosum L.) tuber dormancy release under low-temperature stress based on omics data

DU Xuanrong1,2 , ZHANG Ning1,2 , SI Huaijun1,2   

  1. 1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2026-06-18 Published:2026-06-16

摘要： 为探究低温胁迫下马铃薯块茎休眠解除的遗传调控机制，本文以马铃薯品种‘Favorita’为试验材料，通过对收获后第7天、第30天以及第45天的块茎芽眼部分进行转录组与蛋白质组测序并进行联合分析。以室温环境（22±2）℃作为对照，在低温（4 ℃）胁迫下，收获后第7天有3224个下调的差异基因（DEGs），202个下调的差异蛋白质（DEPs）；收获后第30天有3185个下调的DEGs，468个下调的DEPs；收获后第45天有4763个下调的DEGs，942个下调的DEPs。对3个时期的DEGs进行GO与KEGG富集分析，结果表明，低温可能通过调控能量代谢或植物激素信号通路等途径影响马铃薯的休眠解除。对联合分析的数据结果进一步进行筛选，获取4 ℃条件下均显著下调的DEGs和DEPs，对3个时期的数据进行交叉比对，结果显示在低温胁迫下马铃薯休眠解除过程中第30天到第45天共有27个共下调表达模式的DEGs和DEPs，其中基因ID与蛋白ID一致的有13对。再基于皮尔逊相关性系数筛选出强相关的6对基因—蛋白对，进一步通过qRT-PCR验证了6个核心基因的表达模式，深入讨论了这6对基因—蛋白对在马铃薯休眠解除过程中的功能和作用。研究结果为解析低温胁迫下马铃薯块茎休眠解除的分子网络提供了研究基础。

关键词: 休眠解除, 表达分析, 生物信息学分析, 转录组学, 蛋白质组学

Abstract: To explore the regulatory mechanism of tuber dormancy release in potato under low-temperature stress, this study used the potato cultivar ‘Favorita’ as the experimental material. Transcriptomic and proteomic sequencing and joint analysis were performed on tubers at 7 days, 30 days, and 45 days after harvest. Using room temperature (22±2) ℃ as the control, under low-temperature (4 ℃) stress, there were 3224 down-regulated differentially expressed genes (DEGs) and 202 down-regulated differentially expressed proteins (DEPs) on the 7th day after harvest; 3185 down-regulated DEGs and 468 down-regulated DEPs on the 30th day after harvest; and 4763 down-regulated DEGs and 942 down-regulated DEPs on the 45th day after harvest. GO and KEGG enrichment analyses of DEGs showed that low temperature might affect potato dormancy release by regulating energy metabolism or plant hormone signaling pathways. The results of joint analysis were further filtered for DEGs and DEPs that were significantly down-regulated under 4 ℃. Cross-comparison of data from the three time points identified 27 co-downregulated DEGs and DEPs during potato dormancy release under low-temperature stress from day 30 to day 45, among which 13 pairs had consistent gene IDs and protein IDs. Furthermore, 6 gene-protein pairs with strong correlation were screened out based on the Pearson correlation coefficient. The expression patterns of 6 core genes were verified by qRT-PCR to further analyze the functions of these 6 gene-protein pairs in potato dormancy release. The results provided a basis for deciphering the molecular network of potato tuber dormancy release under low-temperature stress.

Key words: dormancy release, expression analysis, bioinformatics analysis, transcriptomics, proteomics

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