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铁皮石斛SERK基因的鉴定与表达分析

  

  1. 西南交通大学 生命科学与工程学院,成都 610031
  • 出版日期:2019-04-18 发布日期:2019-04-18
  • 通讯作者: 王万军,教授,主要研究方向为植物发育生物学研究,E-mail: wanjunwang@home.swjtu.edu.cn
  • 作者简介:梁易,硕士研究生,研究方向为植物原球茎发育,E-mail:liangyi0711@qq.com
  • 基金资助:
    国家自然科学基金项目 (NO.31371232, NO.31271302)

Identification and expression analysis of SERK gene in Dendrobium officinale Kimura et Migo

  1. College of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
  • Online:2019-04-18 Published:2019-04-18

摘要: 体细胞胚发生相关类受体蛋白激酶(Somatic embryogenesis receptor-like kinase, SERK)广泛存在于植物界中,具有多重功能。通过同源比对方法从铁皮石斛scaffold数据中鉴定出2个SERK基因,命名为DoSERK1和DoSERK2。其中DoSERK2的CDS长为1877 bp,编码628个氨基酸,具有典型的SERK蛋白结构。Conserved domains预测发现DoSERK2胞内激酶活性位点在其三维结构凹陷区。定量表达分析显示DoSERK2在原球茎发育各个时期均有表达,在原球茎发育的第一阶段表达量最高;组织表达分析显示DoSERK2在花中表达量最高,在其他组织器官也有表达,表明该基因可能在早期胚胎发育和生殖过程有重要作用。

关键词: 铁皮石斛, SERK, 原球茎发育, 表达分析

Abstract: Somatic embryogenesis receptor-like kinase (SERK) is widely found in the plant community and has a variety of functions. Two SERK genes were identified from the genomic scaffold data by BLAST alignment in Dendrobium officinale Kimura et Migo, named DoSERK1 and DoSERK2. DoSERK1 has been reported, encoding 633 amino acids; but DoSERK2 is a new SERK gene. The coding sequence(CDS) length of DoSERK2 is 1877 bp, encoding 628 amino acids; DoSERK2 has the typical structure of SERK protein; it has five leucine-rich repeat(LRR) regions, one transmembrane region, one SPP motif and one intracellular kinase area. Conserved domains analysis finds that the active area of its intracellular kinase is in the depression of DoSERK2 three-dimensional structure. Quantitative analysis showed that DoSERK2 expressed in all eight stages of protocorm development, the highest expression level was in the first stage. Tissue expression analysis showed that the DoSERK2 expression was highest in flower, but in other tissues and organs it also could be detected. The gene expression profile indicated that DoSERK2 may play an important role in the early embryo development and reproductive process.

Key words: Dendrobium officinale Kimura et Migo, SERK, protocorm development, expression analysis

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