生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (6): 31-.doi: 10.3969/j.issn.2095-1736.2024.06.031

• 研究报告 • 上一篇    下一篇

不同浓度的四环素对秀丽隐杆线虫肠道菌群中抗生素抗性基因扩散效率的影响

笪艳梅1,2， 张　颖1,2， 李顺顺1,2， 高泽萍1,2， 黄　涛1,2， 孙庆业1,2， 周国伟1,2   

  1. 1. 安徽大学 资源与环境工程学院， 合肥 230601；
    2. 湿地生态保护与修复安徽省重点实验室（安徽大学）， 合肥 230601
  • 出版日期:2024-12-18 发布日期:2024-12-16
  • 通讯作者: 周国伟，博士，副教授，研究方向为微生物生态学，E-mail:gwzhou@ahu.edu.cn
  • 作者简介:笪艳梅，硕士研究生，研究方向为微生物对ARGs传播的影响，E-mail:X21201041@stu.ahu.edu.cn
  • 基金资助:
    国家自然科学基金项目（42107143）

Antibiotic-resistant genes dissemination in the intestinal flora of Canorhabditis elegans in response to antibiotic pressure#br#

DA Yanmei1,2, ZHANG Ying1,2, LI Shunshun1,2, GAO Zeping1,2, HUANG Tao1,2,SUN Qingye1,2, ZHOU Guowei1,2   

  1. 1. College of Resource and Environmental Engineering, Anhui University, Hefei 230601, China; 2. Anhui
    Province Key Laboratory of Wetland Ecosystem Protection and Restoration, Anhui University, Hefei 230601, China
  • Online:2024-12-18 Published:2024-12-16

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摘要： 以秀丽隐杆线虫为模式生物，结合定量PCR和平板筛选法证明抗性基因（ARGs）供体菌Escherichia coliMG1655和受体菌Salmonella typhimurium都能在肠道中稳定定殖。通过接合获得抗生素抗性的S.typhimurium数量与肠道总菌数的比值用以体现肠道中ARGs的扩散效率。在3 d的培养中，随着四环素浓度（0、0.08、0.4、2、10和50 mmol/L）的升高，ARGs扩散效率先升高后降低，其在0.4 mmol/L四环素处理组中达到峰值（第2和第3天分别为0.78%和1.96%）。并且，接合相关功能基因trfA/16S rRNA和trbB/16S rRNA的相对丰度与肠道组织中抗氧化酶活性显现出与ARGs扩散效应一致的趋势。研究表明，短暂且低浓度的抗生素可促进动物肠道内ARGs的扩散。

关键词: 秀丽隐杆线虫, 抗性基因, 质粒接合, 四环素, 肠道微生物

Abstract: Cryptobacterium hidradiiused as a model organism, qPCR was combined with plate screening methods to demonstrate that the antibiotic-resistant genes (ARGs) donor bacteriumEscherichia coliMG1655 and the acceptor bacteriumSalmonella typhimuriumwere both able to colonize stably in the intestine. The ratio of the number of antibiotic-resistantS.typhimuriumobtained via conjugation to the number of total intestinal bacteria was used to characterize the diffusion efficiency of ARGs in the intestine. The ARG diffusion efficiency increased and then decreased with increasing tetracycline concentrations (0, 0.08, 0.4, 2, 10, and 50 mmol/L) over three days of incubation, and it peaked at 0.78% and 1.96% on the second and third days in the 0.4 mmol/L tetracycline-treated group, respectively. Moreover, the relative abundance of conjugation-relevant functional genestrfA/16S rRNAandtrbB/16S rRNAas well as activities of antioxidant enzyme in intestinal tissues shared similar trends with the ARG diffusion ratios in the gut of snails. Therefore, the present study demonstrated that a brief and low concentration of antibiotics could promote the diffusion of ARGs in the animal intestine.

Key words: Canorhabditis elegans, antibiotic-resistant gene, plasmid conjugation, tetracycline, intestinal microorganisms

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