生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 82-.doi: 10.3969/j.issn.2095-1736.2021.06.082

• 研究报告 • 上一篇    下一篇

肝硬化患者肠道微生物与生化指标的关联性

  

  1. 1. 中国科学院烟台海岸带研究所，烟台 264003；2. 山东省烟台市奇山医院，烟台 264001；3. 河海大学 海洋学院，南京 201198；4. 中国科学院海洋大科学研究中心，青岛 266071
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 秦松，研究员，博士，研究方向为海洋生物学，E-mail：sqin@yic.ac.cn
  • 作者简介:李莉莉，博士，副研究员，专业方向为肠道微生物组学，E-mail：llli@yic.ac.cn
  • 基金资助:
    中国科学院青年创新促进会人才项目（2018246）；烟台市科技计划项目（2020MSGY076）

Correlation analysis between gut microbiota and biochemical indicators of cirrhosis

  • Online:2021-12-18 Published:2021-12-15

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摘要： 为揭示肝硬化患者的特征肠道微生物，对12名肝硬化患者(CIR组)及11名健康志愿者(CN组)血液生化指标和肠道微生物组进行了分析。结果发现：与CN组相比，CIR组INR、GGT、TBA、TBIL水平显著上升，同时，ALB、WBC水平显著下降；与CN组相比，CIR组普雷沃氏菌属(Prevotella)、瘤胃菌科UCG-014属(Ruminococcaceae UCG-014)丰度显著上升，布劳特氏菌属(Blautia)、罕见小球菌属(Subdoligranulum)、瘤胃球菌属2(Ruminococcus 2)显著下降；在种水平上，普雷沃氏菌9、瘤胃菌科UCG-014菌丰度显著上升，瘤胃球菌属 5 1 39BFAA菌丰度显著下降。生化指标与肠道菌群的相关性分析表明，在属水平上，普雷沃氏菌9与INR、TBA、GGT显著正相关，与ALB水平显著负相关；瘤胃菌科UCG-014属与INR、GGT水平显著正相关；布劳特氏菌与ALB水平显著正相关，与INR、TBA、TBIL、GGT水平显著负相关；罕见小球菌属与瘤胃球菌属2与WBC水平显著正相关，与TBA、INR水平显著负相关。在种水平上，瘤胃球菌属5 1 39BFAA菌与ALB水平显著正相关，与INR、TBA、TBIL、GGT水平显著负相关。总之，肝硬化组中普雷沃氏菌属，布劳特氏菌属，瘤胃菌科UCG-014属，罕见小球菌属，瘤胃球菌属5 1 39BFAA菌与健康组的差异显著，且这些菌与肝硬化患者的肝损伤相关，这些特征菌群的发现有望为肝硬化的精准治疗提供潜在靶点。

关键词: 肝硬化, 肠道微生物, 生化指标, 肝损伤

Abstract: In order to characterize the gut microbiota of patients with cirrhosis and reveal the association between gut microbiota and biochemical indicators, gut microbiota and biochemical indicators of 12 cirrhosis patients (CIR group) and 11 healthy volunteers (control, CN group) were analyzed. Compared with that of the CN group, the levels of INR, GGT, TBA and TBIL in the CIR group increased significantly, while the levels of ALB and WBC decreased significantly. Gut microbiota analysis indicated that the abundance of genus Prevotella and Ruminococcaceae UCG-014 increased significantly, while genus Blautia, Subdoligranulum and Ruminococcus 2 decreased significantly in the CIR group. At the species level, the abundance of Prevostella 9 and Ruminococcaceae UCG-014 increased significantly, while Ruminococcus sp. 5 1 39BFAA decreased significantly in the CIR group. The correlation analysis between biochemical indexes and intestinal flora showed that the abundance of Prevotella 9 genus had significantly positive correlation with INR, TBA and GGT levels, and negative correlation with ALB level. The abundance of Ruminococcaceae UCG-014 genus was positively correlated with INR and GGT levels. The abundance of Blautia genus demonstrated significantly positive association with ALB level, and negative association with INR, TBA, TBIL and GGT levels. The abundance of Subdoligranulum and Ruminococcus 2 genera exhibited significantly positive correlation with WBC level and negative correlation with TBA and INR levels. At the species level, the abundance of Ruminococcus sp.5 1 39BFAA showed significantly positive correlation with ALB level, and significantly negative correlation with INR, TBA, TBIL and GGT levels. In summary, cirrhosis led to abnormal biochemical indexes of liver and imbalance of intestinal flora. Prevotella genus, Blautia genus, Ruminococcaceae UCG-014 genus, Subdoligranulum genus and Ruminococcus sp.5 1 39BFAA in patients with cirrhosis were significantly different from the healthy group, and these bacteria were related to liver damage. The discovery of these characteristic bacteria and their relationship with biochemical indexes were expected to provide potential targets for precise treatment of cirrhosis.

Key words: cirrhosis, gut microbiota, biochemical indicators, liver damage

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