生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (6): 26-.doi: 10.3969/j.issn.2095-1736.2024.06.026

• 研究报告 • 上一篇    下一篇

儿茶素减轻小鼠肺泡巨噬细胞炎症反应的作用及机制研究

陈淑珍1,2， 许秋凤3， 王皇斌2， 黄莎莎3， 黄丽端3， 熊　闽3   

  1. 1. 福建医科大学 公共卫生学院， 福州 350122； 2. 厦门医学院 基础医学部
    机能与临床转化福建省高校重点实验室， 厦门 361023； 3. 厦门医学院 药学系， 厦门 361023
  • 出版日期:2024-12-18 发布日期:2024-12-16
  • 作者简介:陈淑珍，博士，副教授，研究方向为炎症免疫及免疫毒理，E-mail:cszlotus@126.com
  • 基金资助:
    福建省自然科学基金面上项目（2021J01343）； 厦门医学院科技计划项目（K2023-24）

Study on the anti-inflammatory effect and mechanism of catechin on mouse alveolar macrophages

CHEN Shuzhen1,2, XU Qiufeng3, WANG Huangbin2, HUANG Shasha3,HUANG Liduan3, XIONG Min3   

  1. 1. School of Public Health, Fujian Medical University, Fuzhou 350122, China; 2. Fujian Provincial Key Laboratory
    of Functional and Clinical Translational Medicine, Department of Basic Medicine, Xiamen Medical College,
    Xiamen 361023, China; 3. Department of Pharmacy, Xiamen Medical College, Xiamen 361023, China
  • Online:2024-12-18 Published:2024-12-16

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摘要： 研究旨在探讨比较儿茶素和表儿茶素在小鼠肺泡巨噬细胞（MH-S）中的抗炎作用及其机制。利用脂多糖（LPS）刺激MH-S细胞建立炎症模型，用MTS法和LDH法检测儿茶素和表儿茶素对MH-S细胞活力的影响；ELISA法检测细胞上清液IL-1β、IL-6和TNF-α含量；qRT-PCR 法检测细胞Nos2、Il-6、Tnf-α、Grp78、Ire1α、Perk和Atf6mRNA 表达；Western Blot 法检测iNOS 蛋白表达。结果显示，6.25~100 μg/mL儿茶素和表儿茶素无显著细胞毒性，可浓度依赖地下调LPS诱导的IL-1β分泌和iNOS表达，还降低iNOS、IL-6以及内质网应激（ERS）相关蛋白IRE1α的mRNA转录水平。低浓度儿茶素的抗炎活性强于表儿茶素，高浓度则相反。研究表明，儿茶素和表儿茶素可以通过减少IL-1β、IL-6分泌，抑制iNOS蛋白表达发挥抗炎作用，其抗炎作用可能与抑制ERS有关。

关键词: 儿茶素, 表儿茶素, MH-S细胞, 炎症, 内质网应激

Abstract: The purpose of this study was to explore and compare the anti-inflammatory effects and the possible mechanisms of catechin and epicatechin in mouse alveolar macrophages（MH-S）. Lipopolysaccharide（LPS）was used to stimulate MH-S cells to establish a cellular inflammation model. The effects of catechin and epicatechin on MH-S cell viability were detected by MTS and LDH assays. The contents of interleukin-1β（IL-1β）, IL-6, and TNF-α in the supernatant were detected by enzyme-linked immunosorbent assay（ELISA）. Real-time quantitative polymerase chain reaction（qRT-PCR） was used to detect the mRNA transcription levels ofNos2,Il-6, Tnf-α,Grp78, Ire1α, Perk, andAtf6. The protein expression of iNOS was detected by Western Blot. The results showed that 6.25－100 μg/mL of catechin and epicatechin had no significant toxicity on MH-S cells. Both catechin and epicatechin down-regulated the secretion of IL-1β and the protein expression of iNOS in LPS-induced MH-S cells in a concentration-dependent manner. Meanwhile, catechin and epicatechin reduced the mRNA transcription levels of iNOS, IL-6 and endoplasmic reticulum stress（ERS）-related protein IRE1α. The anti-inflammatory activity of catechin was stronger than that of epicatechin at low concentrations, but catechin was lower than epicatechin at high concentrations. These results indicated that catechin and epicatechin had anti-inflammatory effects by reducing the secretion of IL-1β, IL-6 and the expression of iNOS, which might be related to the inhibition of ERS.

Key words: catechin, epicatechin, MH-S cells, inflammation, endoplasmic reticulum stress

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