生物学杂志 ›› 2021, Vol. 38 ›› Issue (3): 26-.doi: 10.3969/j.issn.2095-1736.2021.03.026

• 神经生物学专题 • 上一篇    下一篇

人参皂苷Rg1对慢性应激小鼠抑郁样行为、海马突触蛋白及胶质细胞的作用

  

  1. 安徽中医药大学新安医学教育部重点实验室,合肥230038
  • 出版日期:2021-06-18 发布日期:2021-06-21
  • 通讯作者: 朱国旗,博士,研究员,博士生导师,研究方向为神经精神疾病发病机理及中医药防治, E-mail:guoqizhu@gmail.com
  • 作者简介:王 娟,硕士研究生,研究方向为神经药理学,E-mail:18709852139@qq.com
  • 基金资助:
    国家自然科学基金项目(81673716);安徽省杰出青年基金项目(1808085J15);安徽中医药大学校级科研项目(2019zryb09);安徽省高校优秀拔尖人才培育资助项目(2020)

Effects of ginsenoside Rg1 on depression-like behaviors, expression of hippocampal synaptic proteins and activation of glial cells in stressed mice

  1. Key Laboratory of Xin′an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China
  • Online:2021-06-18 Published:2021-06-21

摘要: 采用慢性温和不可预期应激(Chronic unpredictable mild stress, CUMS)模型评价人参皂苷Rg1[10、20、40 mg/(kg·d),ip, 7 d]对抑郁样行为的保护作用,旨在探索人参皂苷Rg1对应激小鼠抑郁样行为、海马突触蛋白及胶质细胞活化的影响。结果显示:和正常组比较,CUMS组小鼠糖水偏好指数下降,强迫游泳和悬尾实验中不动时间延长(P<0.05),而给予人参皂苷Rg1[20、40mg/(kg·d)]可降低小鼠抑郁样行为(vs CUMS,P<0.05);CUMS组小鼠海马突触相关蛋白PSD95(Postsynaptic density protein 95)、Arc(Activity-regulated cytoskeleton-associated protein)和BDNF(brain-derived neurotrophic factor)的表达下降,星形胶质细胞标记物GFAP(Glial fibrillary acidic protein)、小胶质细胞标记物Iba1(Ionized calcium binding adapter molecule 1)、炎症反应因子NF-κB(Nuclear factor kappa-B)表达上升(P<0.05)。而给予人参皂苷Rg1能显著增加海马突触相关蛋白的表达,并抑制海马星形胶质细胞和小胶质细胞的活化以及NF-κB的表达(vs CUMS,P<0.05)。表明人参皂苷Rg1可保护CUMS诱导的小鼠抑郁样行为,机理与调控海马突触相关蛋白表达和抑制炎症反应相关。

关键词: 抑郁, 人参皂苷Rg1, 突触可塑性, 炎症反应

Abstract: he aim of this study was to explore the effects of ginsenoside Rg1 on depression-like behaviors, hippocampal synaptic proteins and glial cell activation in stressed mice. Chronic unpredictable mild stress (CUMS) mice model was used to evaluate the effect of ginsenoside Rg1 [10, 20, 40 mg/(kg·d), ip, 7 d] on depression-like behaviors. The results showed that compared with control mice, sucrose preference index of CUMS mice decreased, immobility times was prolonged in the forced swimming and tail suspension experiments, which was prevented by Rg1 pretreatment. The expression of synaptic-related proteins PSD95 (postsynaptic density protein 95), Arc (activity-regulated cytoskeleton-associated protein) and BDNF (brain-derived neurotrophic factor) in the hippocampus of CUMS mice decreased, while the expression levels of astrocyte marker GFAP (glial fibrillary acidic protein), microglial marker Iba1 (ionized calcium binding adapter molecule 1) and inflammatory response factor NF-κB (nuclear factor kappa-B) increased. Administration of ginsenoside Rg1 [20, 40 mg/(kg·d)] increased the expression of hippocampal synaptic proteins, inhibited the activation of hippocampal astrocytes and microglia, and reduced the nuclear factor-κB (NF-κB) expression (vs CUMS, P<0.05). These data implicated that ginsenoside Rg1 prevents the depression-like behaviors likely through increasing the expression of hippocampal synaptic proteins and reducing activation of glial cells in CUMS mice.

Key words: depression, ginsenoside Rg1, synaptic plasticity, inflammation

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