生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 92-.doi: 10.3969/j.issn.2095-1736.2023.04.092

• 综述与专论 • 上一篇    下一篇

常压室温等离子体技术在微生物诱变育种中的研究进展

王泽民1， 晋　昕1， 张飞燕1， 司怀军1   

  1. 1. 上海市农业科学院食用菌研究所 农业农村部南方食用菌资源利用重点实验室 国家食用菌工程技术
    研究中心 上海市农业遗传育种重点实验室， 上海 201403； 2. 上海海洋大学 食品学院， 上海 201306
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 通讯作者: 杨慧，硕士研究生，实验师，研究方向为食用菌育种与菌种保藏，E-mail:huihuiy@163.com
  • 作者简介:陆欢，硕士，助理研究员，研究方向为食用菌育种，E-mail:luhuan@saas.sh.cn;沈玲，在读硕士研究生，研究方向为食用菌育种，E-mail:2948942600@qq.com; 陆欢和沈玲为共同第一作者
  • 基金资助:
    上海市科技兴农项目\[沪农科创字(2020)第1-3号\]

Application of atmospheric and room temperature plasma mutagenesis in microbial and edible fungi mutation breeding

WANG Zemin1, JIN Xin1, ZHANG Feiyan1, SI Huaijun1   

  1. 1. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources
    and Utilization(South), Ministry of Agriculture and Rural Affairs, P. R．China National Engineering Research Center
    of Edible Fungi; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China;
    2. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 主要阐述常压室温等离子体诱变（ARTP）技术的概念、诱变机理和影响诱变效果的因素，以微生物和食用菌诱变育种为主要内容，重点介绍ARTP诱变技术在细菌、放线菌和酵母等微生物菌种改良和提升生物合成能力方面的应用，以及在食用菌新品种选育等研究中的应用，分析ARTP诱变技术在微生物和食用菌育种研究中具有的优势以及面临的挑战，并说明今后研究重点是通过提高快速筛选目标菌株的技术水平，结合高通量测序、转录组和蛋白质组等多组学技术，深入探究经ARTP诱变后微生物和食用菌的遗传规律和调控生物活性物质合成的机制，以期通过新技术的建立和应用，为食用菌和微生物育种研究提供新思路，进而推动微生物和食用菌的种质创新及产业可持续发展。

关键词: 常压室温等离子体, 诱变育种, 微生物, 食用菌, 应用

Abstract: The concept, mutagenesis mechanism and factors affecting the mutagenesis effect of atmospheric and room temperature plasma (ARTP) mutagenesis were mainly described. The application of ARTP mutagenesis technology in improving microbial strains such as bacteria, actinomycetes and yeast and improving their biosynthesis ability, as well as in breeding new strains of edible fungi, was introduced in this paper with the main contents of mutagenesis breeding of microorganisms and edible fungi as the main contents. The advantages and challenges of ARTP mutagenesis technology in the breeding of microorganisms and edible fungi were analyzed. It also showed that the research focuses in the future were to improve the technical level of rapid screening of target strains combined with high-throughput sequencing, transcriptome and proteomics, to deeply explore the genetic law of microorganisms and edible fungi mutated by ARTP and the mechanism of regulating the synthesis of bioactive substances. It was hoped that the result would provide new ideas for the research of edible fungi and microbial breeding through the establishment and application of new technologies, and then promote the germplasm innovation and industrial sustainable development of microorganisms and edible fungi.

Key words: atmospheric and room temperature plasma, mutation breeding, microorganism, edible fungi, application

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