生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 48-.doi: 10.3969/j.issn.2095-1736.2023.04.048

• 研究报告 • 上一篇    下一篇

基于线粒体16S rRNA基因序列探讨中气门亚目的系统发育关系

杨慧娟， 陈　婷， 董文鸽   

  1. 大理大学 病原与媒介生物研究所 云南省自然疫源性疾病防控技术重点实验室, 大理 671000
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 通讯作者: 董文鸽，博士，副研究员，主要研究方向为病原生物学，E-mail:dongwenge2740@sina.com
  • 作者简介:杨慧娟，硕士，主要研究方向为病原生物学，E-mail:1024251899@qq.com
  • 基金资助:
    国家自然科学基金项目(32060143)； 云南省重大科技计划专项(202102AA310056)

Phylogenetic relationship of Mesostigmata based on the mitochondrial 16S rRNA gene sequence

YANG Huijuan, CHEN Ting, DONG Wenge   

  1. Yunnan Provincial Key Laboratory for Zoonosis Control, Institute of Pathogens and Vectors, Dali University,
    Dali 671000, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 为了解中气门亚目物种线粒体16S rRNA基因全序列信息及系统发育关系，采用PCR方法对粪堆寄螨（Parasitus fimetorum）的线粒体基因组进行测序，并结合NCBI数据库中中气门亚目多数类群的序列信息。利用生物信息学软件对中气门亚目物种的线粒体16S rRNA基因进行比较分析，进一步探讨中气门亚目物种间的系统发育及亲缘关系。结果表明：中气门亚目物种线粒体16S rRNA基因的AT含量远高于GC含量；遗传距离在0.111~0.359，平均遗传距离为0.291。碱基转换与颠换比值以及序列饱和性分析显示，中气门亚目物种线粒体16S rRNA基因具有较大的进化潜能，适合用于系统发育分析。使用ML法和BI法构建的系统发育树显示寄螨科与土革螨科形成姐妹分支，与传统分类结果存在差异；大部分同科的物种总是优先聚在一起，表明线粒体16S rRNA基因用于构建中气门亚目的系统发育关系具有稳定、可靠的特点。结果为后续研究中气门亚目物种的系统发育关系奠定基础。

关键词: 中气门亚目, DNA条形码, 线粒体基因组, 16S rRNA, 系统发育

Abstract: To understand the complete sequence information of mitochondrial 16S rRNA gene and the phylogenetic relationships of Mesostigmata species, the mitochondrial genome of Parasitus fimetorum was sequenced by PCR and combined with the sequence information of most taxa of Mesostigmata in the NCBI database. A comparative analysis of mitochondrial 16S rRNA genes of Mesostigmata species was performed using bioinformatics software to further explore the phylogeny and relation among Mesostigmata species. The results showed that the AT content of the mitochondrial 16S rRNA genes of Mesostigmata species was much higher than the GC content; the genetic distance ranged from 0.111 to 0.359, with an average genetic distance of 0.291. The base conversion to inversion ratio and sequence saturation analysis showed that the mitochondrial 16S rRNA genes of Mesostigmata species had a high evolutionary potential suitable for phylogenetic analysis. The phylogenetic trees constructed using ML and BI methods showed that Parasitidae formed sister branches with the Ologamasidae, which differed from the traditional taxonomic results. Most species of the same family always preferentially clustered together, indicating that the mitochondrial 16S rRNA gene was used to construct phylogenetic relationships of Mesostigmata with stable and reliable characteristics. The above results provided a foundation for the subsequent study of the phylogenetic relationships of Mesostigmata species.

Key words: Mesostigmata, DNA barcoding, mitochondrial genome, 16S rRNA, phylogeny

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