生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (3): 63-.doi: 10.3969/j.issn.2095-1736.2023.03.063

• 研究报告 • 上一篇    下一篇

升金湖不同栖息地豆雁肠道菌群组成与特征分析

石水琴， 何光宇， 余　淏， 韩　钰， 张静静， 赵　凯   

  1. 安庆师范大学 生命科学学院 皖西南生物多样性研究与生态保护安徽省重点实验室， 安庆 246000
  • 出版日期:2023-06-18 发布日期:2023-06-19
  • 通讯作者: 赵凯，博士，副教授，硕士研究生导师，主要从事生态学研究方向研究，E-mail: anhui0511@126.com
  • 作者简介:石水琴，博士，讲师，硕士研究生导师，主要从事肠道微生态方向研究，E-mail: shishuiqin0511@163.com
  • 基金资助:
    安徽省自然科学青年基金项目（2108085QC138）； 安徽省重点研究与开发计划项目（202104f06020024）； 安徽省重点实验室开放基金项目（Wy2021001）； 国家级创新创业项目（202110732044X）

Composition and characteristics of intestinal flora of Anserfabalis in different habitats of Shengjin Lake

SHI Shuiqin, HE Guangyu, YU Hao, HAN Yu, ZHANG Jingjing, ZHAO Kai   

  1. Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, School of Life Sciences, Anqing Normal University, Anqing 246000, China
  • Online:2023-06-18 Published:2023-06-19

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摘要： 采用16Sr RNA测序方法对升金湖杨峨头（SLY）和赤岸（SLC）栖息地越冬豆雁肠道微生物组成与特征进行分析。结果表明，升金湖SLY和SLC两个栖息地豆雁肠道微生物组菌群丰度存在显著差异（P＜0.05），SLY以厚壁菌门和变形菌门为优势菌门，SLC以厚壁菌门和蓝细菌门为优势菌门。升金湖SLY栖息地豆雁肠道菌群优势菌属为乳酸杆菌属（39.5%），SLC栖息地豆雁肠道菌群中以Chloroplast属（35.3%）和乳酸杆菌属（19.2%）更为优势。基于Bray_curtis距离矩阵的NMDS分析表明两栖息地菌群呈现出明显分类群。SLC栖息地豆雁肠道菌群网络相关性相比较SLY表现较为紧密和复杂。此外，PICRUSt的功能预测表明，SLY和SLC栖息地豆雁肠道微生物组COG功能注释结果主要为功能预测功能、氨基酸转运和代谢复制功能、重组和修复功能、信号转导机制功能以及能量生产和转换功能等。结果表明，越冬初期升金湖两栖息地豆雁肠道菌群组成存在显著差异，且表现出特有菌群特征，为后续整个越冬期候鸟的研究和保护提供了基础数据和研究方向。

关键词: 升金湖, 不同栖息地, 16S rRNA扩增子测序, 肠道菌群

Abstract: 16S rRNA high-throughput sequencing was used to analyze the intestinal microbial structure and characteristics of Anserfabalis in Yangetou (SLY) and Chi’an (SLC) habitats of Shengjin Lake. The results showed that there was a significant difference in the abundance of intestinal microbiome flora between soybean geese in the two habitats of SLY and SLC in Shengjin Lake (P< 0.05). Firmicutes and Proteobacteria were the dominant bacteria in SLY, and Firmicutes and cyanobacteria were the dominant bacteria in SLC. Lactobacillus (39.5%) was the dominant bacteria in the intestinal flora of Anserfabalis in Shengjin Lake SLY habitat, while norank was the dominant bacteria in the intestinal flora of Anserfabalis in Shengjin Lake and SLC habitat norank_ f__ norank_ o__ Chloroplast (35.3%) and Lactobacillus (19.2%). Based on Bray_ NMDS analysis of Curtis distance matrix showed that the flora in the two habitats showed obvious taxonomic groups. The correlation of intestinal flora network of Anserfabalis in SLC habitat was closer and more complex than that in SLY. In addition, Picrust’s function prediction showed that the cog function annotation results of the intestinal microbiome of Anserfabalis in SLY and SLC habitats mainly included function prediction function, amino acid transport and metabolic replication function, recombination and repair function, signal transduction mechanism function, energy production and conversion function, etc. The results showed that there were significant differences in the composition of intestinal flora of Anserfabalis in Shengjin Lake amphibious habitat at the beginning of winter, and showed unique flora characteristics. The results provided basic data and research direction for the follow-up research and protection of migratory birds in the whole overwintering period.

Key words: Shengjin Lake, different habitat, 16S rRNA amplicon sequencing, intestinal flora

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