生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (3): 56-.doi: 10.3969/j.issn.2095-1736.2023.03.056

• 研究报告 • 上一篇    下一篇

天然橡胶加工废水中异养硝化好氧反硝化菌株的筛选及脱氮特性

惠　昊1， 李娟娟1， 李　宏1， 唐燕琼1， 彭姝璐1， 燕锡尧2， 张　英2， 刘　柱1   

  1. 1. 海南大学 生命科学学院， 海口 570228； 2. 山东海景天环保科技股份公司， 滨州 256600
  • 出版日期:2023-06-18 发布日期:2023-06-19
  • 通讯作者: 刘柱,教授，主要从事微生物、分子生物学以及生物化学等基础研究，E-mail:zhuliu@hainanu.edu.cn
  • 作者简介:惠昊，硕士研究生，主要研究方向为污水生物脱氮，E-mail:huihao2054@163.com
  • 基金资助:
    海南省重点研发计划项目（ZDYF2021XDNY181）； 山东海景天环保科技股份公司横向课题项目（HD-KYH-2021340）

Isolation, identification and denitrification characteristics of heterotrophic nitrification aerobic denitrification bacteria from natural rubber processing wastewater

HUI Hao1, LI Juanjuan1, LI Hong1, TANG Yanqiong1, PENG Shulu1, YAN Xiyao2,ZHANG Ying2, LIU Zhu1   

  1. 1. School of Life Sciences, Hainan University, Haikou 570228, China; 2. Shandong Haijingtian Environmental
    Technology Co., Ltd., Binzhou 256600, China
  • Online:2023-06-18 Published:2023-06-19

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摘要： 从天然橡胶加工废水中分离筛选出一株异养硝化好氧反硝化菌株JD-7，再根据16S rDNA序列和形态学特征初步鉴定该菌株为芽孢杆菌属。菌株JD-7在48 h内能将水体中氨态氮质量浓度从最初的98.24 mg/L降到8.33 mg/L，去除率达到91.52%，并且菌株同时具有去除硝态氮和亚硝态氮的能力，去除率分别达到44.39%和50.06%。通过响应面实验方法探究菌株不同影响因子（温度、初始pH值、碳氮比）下脱氮能力。结果显示，在温度为30.35 ℃、初始pH值为7.85、碳氮比为6.13的条件下，脱氮能力最强。将菌株投入到不同比例的实际废水进行小试实验确定菌株的实际应用效果，在最优条件下JD-7对实际天然橡胶加工废水的氨氮去除速率可达到131.48 mg/(L·d)。研究表明，菌株JD-7具有较高的脱氮能力，在天然橡胶加工废水处理中具有巨大的应用潜力。

关键词: 异养硝化好氧反硝化, 芽孢杆菌, 天然橡胶加工废水, 脱氮特性, 响应面

Abstract: In order to provide new strains for the natural rubber wastewater treatment process, strains that can effectively remove ammonia nitrogen were isolated and screened from natural rubber processing wastewater. A strain with the best nitrification ability was seleted by comparing the denitrification capacity, named JD-7. Based on 16S rDNA gene sequencing and morphological characteristics, the strain preliminarily identified as Bacillus sp. strain JD-7 can reduce the ammonia nitrogen mass concentration from the initial 98.24 mg/L down to 8.33 mg/L, the nitrogen removal rate reached 91.25% in 48 hours. The strain showed the ability to remove nitrate and nitrite, and the removal rates were 44.39% and 50.06%, respectively. Then, the nitrification ability of the strain under different environmental conditions (temperature, initial pH value and C/N ratio) was studied by response surface method. The results indicated that the strongest nitrification ability was observed with temperature of 30.35 ℃, initial pH of 7.85 and the C/N ratio of 6.13. The strain was put into different proportions of actual wastewater for small-scale experiments to determine the practical application effect of the strain. Under the optimal conditions, the ammonia nitrogen removal rate of JD-7 to the actual natural rubber processing wastewater can reach 131.48 mg/(L·d). Strain JD-7 has highly efficient denitrification ability, which demonstrated that the strain has great application potential for nitrogen removal in the treatment of natural rubber processing wastewater.

Key words: heterotrophic nitrification aerobic denitrification, Bacillus sp., natural rubber processing wastewater, nitrification characteristics, response surface

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