生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 58-.doi: 10.3969/j.issn.2095-1736.2022.05.058

• 研究报告 • 上一篇    下一篇

内生多黏类芽孢杆菌对人参不同部位农药残留降解与皂苷单体累积的影响

  

  1. 1.吉林农业大学 中药材学院，长春 130118； 2.吉林省人参工程技术研究中心，长春 130118
  • 出版日期:2022-10-18 发布日期:2022-10-18
  • 通讯作者: 臧埔，硕士，副教授，硕士生导师，研究方向为药用植物学，E-mail: zangpu@163.com
  • 作者简介:郜玉钢，博士，教授，博士生导师，研究方向为生药学，E-mail: gaoyugang_2006@163.com
  • 基金资助:
    吉林省科技厅医药健康专项项目（20200708025YY）

Effect of endophytes Bacillus polymyxa on degradation of pesticide residues in different parts of ginseng and accumulation of#br# ginsenoside monomers

  1. 1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;
    2. Jilin Province Ginseng Engineering Technology Research Center, Changchun 130118, China)
  • Online:2022-10-18 Published:2022-10-18

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摘要： 为探究多黏类芽孢杆菌对人参不同部位农药残留及人参皂苷含量影响，通过气相色谱法、高效液相色谱法分别对人参内生多黏类芽孢杆菌处理前后人参不同部位农药残留及人参皂苷含量测定，比较其差异。结果显示：多黏类芽孢杆菌对5个部位农残降解率由大到小依次为芦头87.25%、须根69.87%、支根69.02%、木质部54.28%、韧皮部32.69%，对照组各部位农残降解率仅为7.58%~13.33%（P<0.05）。多黏类芽孢杆菌对人参各部位的9种人参皂苷加合值的含量累积效果由大到小依次为须根21.34%、芦头12.00%、支根9.03%、韧皮部8.60%、木质部8.18%（P<0.05）。结果表明，人参不同部位的农药残留与人参皂苷含量均有不同分布，多黏类芽孢杆菌对5个部位农残降解率以及9种人参皂苷的含量影响显著(P<0.05)。

关键词: 人参, 不同部位, 内生多黏类芽孢杆菌, 农药残留, 人参皂苷

Abstract: In order to explore the effect of Bacillus polymyxa on the pesticide residues and ginsenoside content in different parts of ginseng, the pesticide residues and ginsenoside content in different parts of ginseng were determined by gas chromatography and high performance liquid chromatography before and after treatment with Bacillus polymyxa. The results showed that the degradation rates of Bacillus polymyxa on the five parts of pesticide residues in descending order were 87.25% of rhizome, 69.87% of fibrous roots, 69.02% of branch roots, 54.28% of xylem, and 32.69% of phloem. The degradation rate was only 7.58%-13.33% (P<0.05). The cumulative effects of Bacillus polymyxa on the content of the 9 ginsenosides in each part of ginseng in descending order were 21.34% fibrous root, 12.00% rhizome, 9.03% branch root, 8.60% phloem, 8.18% xylem (P<0.05). The results showed that the pesticide residues and ginsenoside content in different parts of ginseng had different distributions. Bacillus polymyxa had a significant effect on the degradation rate of pesticide residues in five parts and the content of 9 ginsenosides (P<0.05).

Key words: ginseng, different part, endophytes Bacillus polymyxa, pesticide residue, ginsenoside

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