生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 65-.doi: 10.3969/j.issn.2095-1736.2022.05.065

• 研究报告 • 上一篇    下一篇

产酸克雷伯氏菌胞内粗酶液降解泰乐菌素

  

  1. 1. 浙江农林大学 环境与资源学院， 杭州 311300； 2. 浙江省土壤污染生物修复重点实验室 浙江农林
    大学环境与资源学院， 杭州 311300； 3. 浙江省农业科学院 环境资源与土壤肥料研究所， 杭州 310021
  • 出版日期:2022-10-18 发布日期:2022-10-18
  • 通讯作者: 郑华宝，博士，教授，研究方向为农林废弃物资源化利用，E-mail: zhenghuabao@zafu.edu.cn
  • 作者简介:张涛，硕士研究生，研究方向为抗生素污染的微生物修复，E-mail: zhangtao9508@163.com
  • 基金资助:
    国家重点研发计划项目（2018YFD0500206）； 浙江省重点研发计划项目（2021C03190）； 浙江农林大学科研发展基金项目（2034020081）； 浙江农林大学大学生科研训练计划项目（2013200154）

Biodegradation of tylosin by intracellular crude enzyme solution of Klebsiella oxytoca

  1. 1. School of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300,
    China; 2. Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, School of Environmental and
    Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China; 3. Institute of
    Environmental Resources and Soil Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Online:2022-10-18 Published:2022-10-18

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摘要： 以产酸克雷伯氏菌TYL-T1胞内粗酶液作为研究对象，研究TYL-T1胞内粗酶液对泰乐菌素的降解特性。当降解体系中含有25 mg/L泰乐菌素时，最适降解条件为pH 7.0、45 ℃和2.5 mg胞内粗酶液投加量，在6 h内泰乐菌素的降解率达到94.33%。分别添加重金属离子Co2+、Fe2+、Mn2+，能够促进泰乐菌素的降解。泰乐菌素酶促降解过程符合一级动力学反应模型，降解速率常数为0.714 9 h-1，半衰期为1.0 h，酶促降解最大反应速率为22.58 mg/(L·h)。降解产物没有抑制指示菌的生长，表明胞内粗酶液能完全消除泰乐菌素的生态学毒性。因此，利用产酸克雷伯氏菌TYL-T1胞内粗酶液处理泰乐菌素的残留具有很好的应用前景。

关键词: 泰乐菌素, 产酸克雷伯氏菌, 胞内粗酶液, 降解动力学, 产物毒性

Abstract: Intracellular crude enzyme solution of Klebsiella oxytoca (TYL-T1) was used as a starting material. The degradation characteristics of tylosin by TYL-T1 intracellular crude enzyme solution were studied. When the degradation system contains 25 mg/L of tylosin, the optimal degradation conditions were pH 7.0, 45 ℃, and 2.5 mg intracellular crude enzyme, resulting in 94.33% degradation rate within six hours. The addition of heavy metal ions such as Co2+, Fe2+, and Mn2+, respectively, promoted tylosin degradation. The process of tylosin degradation conformed to the first-order kinetic reaction model. The degradation rate constant was 0.714 9 h-1, the half-life was 1.0 h, and the maximum reaction rate was 22.58 mg/(L·h). The degradation products did not inhibit the growth of indicator bacteria, indicating that intracellular crude enzyme solution could eliminate the ecological toxicity of tylosin. Therefore, the intracellular crude enzyme solution of Klebsiella oxytoca might become an alternative tool to degrade tylosin.

Key words: tylosin, Klebsiella oxytoca, intracellular enzyme crude solution, degradation kinetics, product toxicity

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