生物学杂志 ›› 2021, Vol. 38 ›› Issue (6): 70-.doi: 10.3969/j.issn.2095-1736.2021.06.070

• 研究报告 • 上一篇    下一篇

高效根结线虫生防真菌筛选及其性能研究

  

  1. 1. 河南省微生物工程重点实验室,郑州 450000;2. 河南省科学院生物研究所有限责任公司,郑州 450000
  • 出版日期:2021-12-18 发布日期:2021-12-15
  • 通讯作者: 王继雯,硕士研究生,研究员,主要从事农业微生物技术研究,E-mail: wangjiwen2005@126.com
  • 作者简介:李磊,硕士研究生,研究实习员,研究方向为农业微生物学,E-mail: lilei649265@126.com
  • 基金资助:
    河南省科学院科技开发补助项目(190305004); 河南省科技攻关项目(182102110036); 河南省科学院科技开发补助项目(200305002)

Screening and performance of high-efficiency root-knot nematodebiocontrol fungi

  1. 1. Henan Provincial Key Laboratory of Microbial Engineering, Zhengzhou 450000, China;
    2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou 450000, China
  • Online:2021-12-18 Published:2021-12-15

摘要: 为了获得具有防治根结线虫作用的菌株,采用几丁质平板透明圈初筛和离体拮抗试验复筛的方法,得到一株具有生防潜力的真菌菌株SFC-3。通过形态学特征观察和真菌内转录间隔区序列(Internal transcribed spacer,ITS)分析鉴定,确定菌株SFC-3为棘孢木霉(Trichoderma asperellum),其几丁质酶活为2.07 U/mL。根结线虫虫卵寄生实验和二龄幼虫致死率实验表明:菌株SFC-3对虫卵的寄生率最高达到78.86%,高于SFC-1、SFC-22等其他9株菌,在第7 天时对根结线虫虫卵孵化的相对抑制率最高达到96.51%,对二龄幼虫的校正死亡率达到93.37%。通过筛选抗根结线虫菌株并研究了其生防效果,证实了菌株SFC-3有比较优秀的抗根结线虫性能。

关键词: 根结线虫, 棘孢木霉, 几丁质酶活, 种质资源, 生物防治

Abstract: In order to obtain strains with the effect of preventing and controlling root-knot nematodes, the chitin flat transparent ring primary screening and the in vitro antagonistic test rescreening methods were used to obtain a fungal strain SFC-3 with biocontrol potential. Through observation of morphological characteristics and analysis and identification of transcribed spacer sequences (ITS) in fungi, the strain SFC-3 was identified as Trichoderma asperellum and its chitinase activity was 2.07 U/mL. The root-knot nematode egg parasitic experiment and the second-instar larval lethality experiment showed that the strain SFC-3 had the highest parasitic rate of 78.86%, which was higher than SFC-1, SFC-22 and other 9 strains. At the 7th day, the relative inhibition rate of egg incubation of root-knot nematodes reached 96.51%, and the corrected mortality rate of second-instar larvae reached 93.37%. In this study, by screening anti-knot nematode strains and studying their biocontrol effects, it was confirmed that the strain SFC-3 had better anti-root knot nematode performance.

Key words: root-knot nematode, Trichoderma echinosporium, chitinase activity, germplasm resources, biological control

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