生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (4): 54-.doi: 10.3969/j.issn.2095-1736.2023.04.054

• 研究报告 • 上一篇    下一篇

高原林蛙线粒体全基因组序列测定及系统发育分析

张湑泽1,2， 董　豹1， 哈金强1， 赵　英1， 魏生楠1， 韦迎婷1   

  1. 1. 青海民族大学 生态环境与资源学院， 西宁 810007；
    2. 青海省特色经济植物高值化利用重点实验室, 西宁 810007
  • 出版日期:2023-08-18 发布日期:2023-08-10
  • 作者简介:张湑泽，硕士，副教授，研究方向为高原两栖动物多样性与进化，E-mail:zxz1904@126.com
  • 基金资助:
    青海省自然科学基金项目（2020-ZJ-965Q）； 青海民族大学校级规划项目（2021XJGH15）； 青海省动物生态基因组学重点实验室开放课题项目（QHEG-2019-04）

Complete mitochondrial genome sequence and phylogenetic analysis of Rana kukunoris

ZHANG Xuze1,2, DONG Bao1, HA Jinqiang1, ZHAO Ying1, WEI Shengnan1, WEI Yingting1   

  1. 1. College of Eco-Environment and Resources, Qinghai Minzu University, Xining 810007, China; 2. Qinghai
    Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants, Xining 810007, China
  • Online:2023-08-18 Published:2023-08-10

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摘要： 利用二代测序技术Illumina平台对高原林蛙（Rana kukunoris）线粒体进行低覆盖全基因组测序，对线粒体基因组进行获取、拼接与注释，获得高原林蛙线粒体全基因组序列，序列全长21913 bp，主要包括tRNA基因（22个）、rRNA基因（2个）、CDS（13个）和D-loop(1个)4部分。除轻链中的NAD6外，其他蛋白质编码基因都在同一条链上。在编码基因中，共发现3个重叠基因：COX1/trnS2、ATP6/ATP8和NAD4L/NAD4。高原林蛙线粒体基因组具有AT偏好性，蛋白质编码基因中氨基酸使用最频繁的是Leu和Ser，tRNA基因都有经典的三叶草结构。KaKs分析显示，高原林蛙线粒体基因组编码基因未检测到正选择作用。基于高原林蛙线粒体全基因组构建的系统发育树表明，高原林蛙与中国林蛙亲缘关系最近。研究结果将为高原林蛙的起源、分化和遗传多样性研究提供数据支持。

关键词: 高原林蛙, 线粒体全基因组, 序列分析, 系统发育, 物种分化

Abstract: The high-throughput sequencing of illumina platform was used to sequence the whole mitochondrial genome of Rana kukunoris. Then, the mitochondrial genome was assembled and annotated. The total length of the sequence was 21913 bp, which mainly included four parts: tRNA gene (22), rRNA gene (2), CDS (13) and D-loop (1). ExceptNAD6in the light chain, other protein coding genes were on the same chain. Of the coding genes, three overlapping genes were found:COX1/trnS2,ATP6/ATP8andNAD4L/NAD4. Mitochondrial genome of Rana kukunoris had AT preference. Leu and Ser were the most frequently used amino acids in protein coding genes, and all tRNA had the classic clover structure. KaKs analysis showed that the coding genes of mitochondrial genome were not significantly positively selected in Rana kukunoris. The phylogenetic tree constructed based on the whole mitochondrial genome showed that Rana kukunoris was closely related to Rana chensinensis. It provided the genetic information of Rana kukunoris mitochondrial genome and aid future investigation of phylogenetics, speciation and evolution of Rana genus.

Key words: Rana kukunoris, mitochondrial genome, sequence analysis, phylogeny, species differentiation

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