生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 95-.doi: 10.3969/j.issn.2095-1736.2023.02.095

• 综述与专论 • 上一篇    下一篇

UDP-糖生物合成的研究进展

陈归航1， 李　春1,2， 冯旭东1   

  1. 1. 北京理工大学 化学与化工学院 生物化工研究所医药分子科学与制剂工程工业和信息化部重点
    实验室， 北京 100081； 2. 清华大学 化学工程系 工业生物催化教育部重点实验室， 北京 100084
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 通讯作者: 冯旭东，副教授，研究方向为酶工程，E-mail:xd.feng@bit.edu.cn
  • 作者简介:陈归航，硕士，研究方向为酶工程，E-mail:2405824672@qq.com
  • 基金资助:
    国家自然科学基金面上项目（21878021， 22178025）

Research progress of UDP-sugar biosynthesis

CHEN Guihang1, LI Chun1,2, FENG Xudong1   

  1. 1. Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering Ministry of Industry and Information
    Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of
    Technology, Beijing 100081, China; 2. Key Lab for Industrial Biocatalysis, Ministry of Education, Department of
    Chemical Engineering, Tsinghua University, Beijing 100084, China
  • Online:2023-04-18 Published:2023-04-18

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摘要： 尿苷二磷酸（UDP）-糖是糖基化修饰利用的一类重要糖供体，从合酶途径、磷酸化酶途径和激酶途径总结UDP-糖的体内合成过程，由于关键酶的缺乏，只有UDP-葡萄糖（UDP-Glc）能从以上3种途径快速合成。通过特定功能酶能够实现UDP-糖之间快速转化，这种转化以UDP-葡萄糖为起始物，以UDP-葡萄糖醛酸（UDP-GlcA）为中间体，在此基础上，概述特定功能酶催化UDP-糖合成的最新进展，探讨脱氢酶、脱羧酶、异构酶和还原酶在UDP-糖转化中的重要作用。最后，剖析UDP-糖合成的现存问题，展望UDP-糖合成的未来研究方向，旨在为更快地挖掘UDP-糖基供体的作用潜能和实现更加高效低成本的糖基化修饰提供新思路。

关键词: UDP-糖, 生物合成, 脱氢酶, 脱羧酶, 异构酶, 还原酶

Abstract: Uridine diphosphate(UDP)-sugar is an important type of sugar donors for glycosylation modification. The in vivo synthesis of UDP-sugar was summarized from three aspects: synthase pathway, phosphorylase pathway and kinase pathway. Due to the lack of key enzymes, only UDP-glucose (UDP-Glc) could be quickly obtained from the above three pathways. At the same time, with UDP-glucose as the starting material and UDP-glucuronic acid (UDP GlcA) as the intermediate, the rapid interconversions between UDP-sugars could be realized by specific functional enzymes. The latest progress in UDP-sugar synthesis catalyzed by specific functional enzymes was reviewed, and the important roles of dehydrogenase, decarboxylase, isomerase and reductase in UDP-sugar interconversions were discussed. The existing problems of UDP-sugar synthesis were analyzed and the future research direction of UDP-sugar synthesis was prospected, aiming at providing new ideas for tapping the potential of UDP-sugar donors and realizing glycosylation modification with efficiency and low cost.

Key words: UDP-sugar, biosynthesis, dehydrogenase, decarboxylase, isomerase, reductase

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