Abstract: The HE staining was used to observe the uterus tissue morphology, the RT-PCR and the immunohistochemistry were adopted to assay the ER levels in ovariecomized rats, and the therapeutic mechanism of Rosa rugosa, Thunb Radix Hedysari, Angelicae Sinensis Radix was explored. The middle dose group, high dose group of Rosa rugosa, Thunb Radix Hedysari and Angelicae Sinensis Radix decoction and estradiol valerate tablet group(positive control group) had significant difference in decreasing the rats weight(241.00±18.81, 236.10±25.20, 236.33±17.95) g, the rats in the middle group, high group and estradiol valerate tablet group showed significant differences in increasing the index of uterus(0.67±0.10, 0.76±0.11, 0.77±0.07); the gene expression and the protein of ERα(1.17±0.13/1367.19±214.32, 1.28±0.15/1478.39±195.62, 1.36±0.09/1279.35±168.33) and ERβ(1.13±0.03/1653.73±170.49, 1.05± 0.07/1833.94±195.45, 1.16±0.16/1713.25±231.73) were also raised at the same time in the rats of middle group, high group and estradiol valerate tablet group; the contents of E2(12.87±3.49, 17.15±4.73, 19.19±3.79, 16.48±3.34) ng/mL were increased in the rats of low group, middle group, high group and estradiol valerate tablet group(P<0.05). The glands appeared and monolayer columnar epithelial cells arranged neatly in high group and estradiol valerate tablet group. The experiment suggested that Rosa rugosa, Thunb Radix Hedysari and Angelicae Sinensis Radix decoction could improve ovariecomized rats from pathological changes of uterus, and the effect might be related to that Rosa rugosa, Thunb Radix Hedysari and Angelicae Sinensis Radix decoction could regulate the contents of E2 in serum and the expression of ERα, ERβ in uterus.

Key words: Rosa rugosa, Thunb Radix Hedysari and Angelicae Sinensis Radix decoction, perimenopause, uterus pathological morphology, estrogen receptor