生物学杂志

Journal of Biology ›› 2020, Vol. 37 ›› Issue (1): 35-.doi: 10.3969/j.issn.2095-1736.2020.01.035

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Effect of N-carbamylglutamate on cell proliferation and gene expression in pig placenta trophoblast cells

  

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070;  2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097;  3. Pingliang Mechanical and Electrical Engineering School of Gansu Province, Jingning 743400; 
    4. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Online:2020-02-18 Published:2020-03-09

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Abstract: tN-carbamylglutamate (NCG) had a positive effect on placental development and function in sow. To reveal the mechanism of NCG regulation on placental function, this research used pig placental trophoblast cells (pTr) as an in vitro model to evaluate the effect of NCG on cell proliferation and related gene expression. The result showed that NCG (1-25 mmol/L) increased pTr cell proliferation 1.2 to 1.5 folds (P<0.05), in which 10 mmol/L NCG upregulated CDK4, CCND1, SLC2A, and CAT gene expressions (P<0.05). The result demonstrated that NCG could stimulate pTr cell proliferation through upregulating genes related with cell cycle, glucose transportation and antioxidant stress processes.

Key words: N-carbamylglutamate, placenta trophoblast cell, cell proliferation, gene expression

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