Abstract: The comprehensive experiment of plant biological breeding is a comprehensive design experiment course integrating crop breeding, molecular biology, genetics, genetic engineering and bioinformatics under the background of new agricultural science. The “Creation of new high-oil soybean germplasm based on CRISPR/Cas9 gene editing technology” is a comprehensive design project in the experimental library. The project takes bioinformatics as the lead, molecular biology and biological breeding as the main body, and crosses over genetic engineering and genetics. It is a new comprehensive experimental project of biological breeding under the background of new agricultural science. The experiment comprised 5 modules, including program design, simulation training, experiment implementation, skill training and practice internship, to help students systematically master cutting-edge technologies such as plant germplasm resource innovation, digital breeding, and genome editing, mobilize learning enthusiasm, realize active learning, and stimulate students’ innovative thinking and feelings toward “agriculture, rural areas and farmers”. This experiment project was taken as an example to explore the reform of the plant biological breeding comprehensive experiment in teaching, aiming to provide a referential experience for biological breeding-related disciplines, further optimizing the curriculum and continuously updating and improving.

Key words: new agricultural science, comprehensive experiment of plant biological breeding, knowledge crossing