Abstract: To explore the mitochondrial genome of Narosa fulgens(Leech, 1889) and the phylogeny of Limacodidae,we newly sequenced the complete mitochondrial genome of N. fulgens(Lepidoptera, Limacodidae), by using the next-generation sequencing method. In phylogenetic analyses, we selected 16 exemplars of Zygaenoidea as ingroups. Each onespecies of Cossidae and Thyrididae was used as outgroup. We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods. The sequenced genome is a circular molecule of 15296 bp in length (GenBank accession No. OP919326) which possesses 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and putative control regions as well. For most of the protein-coding genes, the star codons were ATN except forcox1using CGA as star codon. Most of the protein-coding genes terminated with the stop codon TAA, whilecox2andnad4employed the incomplete T as the stop codon.All tRNA genes can be folded into atypical cloverleaf structure, with the except of thetrnS1-AGN. ThetrnS1lacks the DHU arm.The lengths of the rrnL and rrnS genes were 1335 bp and 732 bp, respectively, with the A + T contents of 85.24% and 84.70%. Both phylogenetic inference methods showed largely identical tree topological structures: the families Limacodidae and Zygaenidae were respectively monophyletic, Narosa fulgens is sister to Narosa nigrisigna.

Key words: Lepidoptera, Limacodidae, Narosa fulgens, mitochondrial genome, phylogeny