活性氧介导非热大气压等离子体杀伤肺癌细胞的机制

doi:10.3969/j.issn.2095-1736.2023.05.001

Journal of Biology ›› 2023, Vol. 40 ›› Issue (5): 1-.doi: 10.3969/j.issn.2095-1736.2023.05.001

The killing mechanism of lung cancer cells by nonthermal atmospheric pressure plasma mediated by ROS

ZHOU Yuanyuan^1,2, LIU Fang^1,2, CHEN Jinwu^2,4, LIU Lei²,SONG Wencheng^2,3,5, CHU Yannan^2,3

1.Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China; 2.Anhui
Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei
Institutes of Physical Science, Chinese Academy of Sciences,Hefei 230031, China; 3. Hefei Cancer Hospital, Chinese
Academy of Sciences, Hefei 230031, China; 4. School of Biology and Food Engineering, Hefei Normal University,
Hefei 230061, China; 5.Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education
Institutions, Suzhou 215123, China

Online:2023-10-18 Published:2023-10-17

Abstract

Abstract: The nonthermal atmospheric plasma (NAP) was used to treat lung cancer cells H460. With the extension of NAP treatment time, MTT assay showed that NAP could decrease the cell viability of H460 significantly, JC-1 assay showed that NAP decreased the mitochondrial membrane potential of H460, which represented the occurrence of cell apoptosis. Western Blot experiment showed that NAP inhibited the expression of PI3K, AKT, JNK and STAT3, demonstrating the killing effect of NAP related to the expression of PI3K/AKT and JNK/STAT3/AKT. Besides, the addition of ROS scavenger NAC effectively eliminated the damage caused by NAP, suggesting that the killing of lung cancer H460 cells by NAP were mediated by ROS, which provided theoretical basis for the feasibility of NAP in the treatment of lung cancer.

Key words: nonthermal atmospheric plasma, lung cancer, H460 cells, ROS, cell apoptosis

CLC Number:

ZHOU Yuanyuan, LIU Fang, CHEN Jinwu, LIU Lei, SONG Wencheng, CHU Yannan. The killing mechanism of lung cancer cells by nonthermal atmospheric pressure plasma mediated by ROS[J]. Journal of Biology, 2023, 40(5): 1-.

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The killing mechanism of lung cancer cells by nonthermal atmospheric pressure plasma mediated by ROS

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