Journal of Biology ›› 2023, Vol. 40 ›› Issue (4): 20-.doi: 10.3969/j.issn.2095-1736.2023.04.020

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Enhancement of phenazine-1-carboxylic acid production for Pseudomonas chlororaphis GP72-ND3ΔphzO by fermentation optimization

LIU Zhenzhen, WANG Wei, HUANG Xianqing, ZHANG Xuehong   

  1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology,
    Shanghai Jiao Tong University, Shanghai 201100, China
  • Online:2023-08-18 Published:2023-08-10

Abstract: The fermentation conditions of this strain were optimized and scaled up in a 5 L bioreactor. First, through single factor experiment, orthogonal experiment, steepest climbing experiment and central composite design experiment at the shake flask level, the optimal medium composition was obtained as 49.55 g/L glycerol, 37.34 g/L soy peptone, 1.5 g/L KCl, 0.75 g/L MgSO4and 0.225 g/L K2HPO4, and the maximum production of phenazine-1-carboxylic acid (PCA) after 60 h fermentation was (6.01±0.17) g/L, which was 2.6 times as that before optimization. Then, the fermentation pH was optimized in 1 L bioreactors. When the pH was maintained at 6.8 during the culture process, the PCA production was the highest at (7.16±0.04) g/L, which was 1.2 times as that before optimization. Finally, under the optimal medium and pH conditions, the strain was scaled up cultured in 5 L bioreactors, and the maximum PCA production after 60 h of fermentation was (6.84±0.53) g/L. This study provided support for improving the production and industrial application of PCA.

Key words: Pseudomonas chlororaphis GP72-ND3ΔphzO, phenazine-1-carboxylic acid, fermentation optimization, central composite design, bioreactor

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