Abstract: Transcriptome sequencing was used to deeply understand the gene expression profile of a strain of Serratia producing red pigment at different growth stages (pigment and non-pigment producing) which was isolated and purified in the laboratory. A total of 2512 differentially expressed genes were obtained from the gene expression profiles of different growth stages, of which 1317 were significantly up-regulated. Pathway enrichment analysis showed that there was a significant enrichment trend in quorum sensing function. In the pigment production stage, the AI-2 kinase lsrk was highly expressed, and the LuxR family transcription regulator sdiA was also highly expressed. And 1195 genes were significantly down regulated, and pathway enrichment analysis showed that there were significant enrichment trends in ribosome, flagellum assembly, TCA cycle, peptidoglycan biosynthesis, aminoacyl tRNA biosynthesis, prokaryotic carbon fixation, cationic antimicrobial peptide resistance, bacterial chemotaxis, oxidative phosphorylation, alanine, aspartate and glutamate metabolism, and bacterial secretion system.