Abstract: SNRPD1is responsible for encodinga protein belonging to the spliceosome family, which is recognized as an essential player in RNA splicing process. It was later found to be implicated in the development of several types of cancer. We used bioinformatics and molecular assays to predict and validate the function ofSNRPD1in breast cancer development. Transcriptome data analysis revealed that higherSNRPD1expression was observed in tumor tissue compared to normal tissue andSNRPD1was highly expressed in Triple Negative subtype, the most malignant subtype of breast cancer. Survival analysis confirmed that patients with higherSNRPD1expression exhibits worse survival rate and higher relapse probability. Correlation analysis showed thatSNRPD1co-expressing genes are majorly involved in cell proliferation pathway and cell division process. Molecular assays confirmed silencingSNRPD1will lead to reduced cell proliferation and under-regulated JNK expression. Our study revealed the function ofSNRPD1in breast cancer development and proposesSNRPD1as a potential breast cancer prognostic marker, which can deliver valuable insights for breast cancer clinical treatment.

Key words: basal breast cancer, cell proliferation, bioinformatics, spliceosome